Biotechnology and Bioprocess Engineering

, Volume 21, Issue 1, pp 39–45 | Cite as

Glucooligosaccharide production by Leuconostoc mesenteroides fermentation with efficient pH control, using a calcium hydroxide-sucrose solution

  • Sun Lee
  • Nguyen Thi Thanh Hanh
  • Jae-Young Cho
  • Ji Youn Kim
  • Young Hwan Moon
  • Su-Cheong Yeom
  • Geun-Joong Kim
  • Doman Kim
Research Paper


95.3% of the sucrose in a feed batch fermentation (300 g/L) was hydrolyzed by Leuconostoc mesenteroides subp. mesenteroides NRRL B-23188 glucansucrase. Further, the glucose of sucrose formed glucooligosaccharides (GOS) of degree of polymerization (DP) over 2, together with 91.6% of the maltose (200 g/L). Lime saccharate (lime sucrate) was used to control the pH during fermentation. The GOS products had DP between 2 and 7. When Streptococcus mutans mutansucrase (0.1 U/mL) reacted with 0.1% sucrose, addition of 0.1 ~ 10% GOS to the mutansucrase reaction digest resulted in a 56 ~ 90% reduction of mutan formation. GOS also reduced E. coli (72.2%) and Salmonella sp. (over 40.0%) growth, when 2.5% GOS was used as a single carbon source, compared to growth using glucose. The calculated glycemic index and glycemic load of GOS was 8 and 1, respectively, based on a 10 g carbohydrate serving. GOS was calculated to have 2.43 kcal/g. After a glucose tolerance test was performed using C57BL/6 mice, we found that mice treated with GOS showed a 59.4% lower increase in plasma glucose than those treated with maltose.


glucansucrase Leuconostoc mesenteroides glucooligosaccharides calcium hydroxide 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Sun Lee
    • 1
    • 2
  • Nguyen Thi Thanh Hanh
    • 1
  • Jae-Young Cho
    • 1
  • Ji Youn Kim
    • 5
  • Young Hwan Moon
    • 3
  • Su-Cheong Yeom
    • 1
    • 5
  • Geun-Joong Kim
    • 4
  • Doman Kim
    • 1
    • 5
  1. 1.Institutes of Green Bio Sciences & TechnologySeoul National UniversityGangwon-doKorea
  2. 2.School of Biological Sciences and TechnologyChonnam National UniversityGwangjuKorea
  3. 3.Audubon Sugar InstituteLouisiana State University Agricultural CenterSaint GabrielUSA
  4. 4.Department of Biological Sciences, College of Natural SciencesChonnam National UniversityGwangjuKorea
  5. 5.Graduate School of International Agricultural TechnologyGangwon-doKorea

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