Flavin mononucleotide mediated microbial fuel cell in the presence of Shewanella putrefaciens CN32 and iron-bearing mineral
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We investigate the workability of electron transfer mediators (ETMs) for the enhanced microbial fuel cell (MFC) operated by Shewanella putrefaciens CN32. In open-circuit, natural ETMs showed 128 ~ 166% of enhancement in the difference of electrical potential compared to that without ETMs (0.41 V), while MFC with synthetic ETMs achieved 200 ~ 250% of enhancement, showing a liner relationship between the electric potentials and ETMs’ redox potentials (R2 = 0.91). Especially, flavin mononucleotide (FMN), the most effective ETM for Shewanella-MFC operation, exhibited the highest potential (0.547 V) and power density (20.28 mW/m2) and generated the maximum current (0.285 mA). The addition of conductive iron-bearing minerals (hematite: 26.27 mW/m2, lepidocrocite: 25.83 mW/m2) enhanced power density of FMN-MFC, while no significant enhancement was observed when soluble iron source (ferric citrate) was added. In addition, MFC containing hematite showed the maximum current (0.37 mA) with 30.8 % of coulombic efficiency.
Keywordsmicrobial fuel cell Shewanella putrefaciens CN32 electron transfer mediator iron-bearing minerals flavin mononucleotide
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- 9.Kim, H. J., M. S. Hyun, I. S. Chang, and B. H. Kim (1999) A microbial fuel cell type lactate biosensor using a metal-reducing bacterium, Shewanella putrefaciens. J. Microbiol. Biotechnol. 9: 365–367.Google Scholar
- 10.Myers, C. R. and J. M. Myers (1992) Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1. J. Bacteriol. 174: 3429–3438.Google Scholar