Enhancement of taxol production from endophytic fungus Fusarium redolens
The optimization of taxol production by Fusarium redolens by one factor at a time (OFAT) approach led to production of 70 μg/L of taxol. With sucrose and NH4NO3 as the carbon and nitrogen sources and medium volume (Vm) to flask volume (Vf) ratio of 0.2, a greater taxol production was attained. NH4NO3, MgSO4↔7H2O and NaOAc at 6.25, 0.63, and 1.25 g/L, were the significant factors for attaining the highest taxol production. The optimization of culture variables led to the production of taxol from 66 to 198 μg/L, which is three fold higher than that in the unoptimized medium. Current study results suggested the success of Response Surface Methodology in enhancing the production of fungal taxol.
Keywordstaxol Fusarium redolens Plackett-Burman design response surface methodology
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