Biotechnology and Bioprocess Engineering

, Volume 17, Issue 5, pp 887–899

Elucidation of bacterial genome complexity using next-generation sequencing

  • Jungkon Kim
  • Sooin Lee
  • HyeonSeok Shin
  • Sun Chang Kim
  • Byung-Kwan Cho
Review Paper

DOI: 10.1007/s12257-012-0374-x

Cite this article as:
Kim, J., Lee, S., Shin, H. et al. Biotechnol Bioproc E (2012) 17: 887. doi:10.1007/s12257-012-0374-x
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Abstract

Next-generation sequencing (NGS) technologies generate higher resolution and less noise data that can allow the assembly of bacterial genome sequences. It also enables the characterization and quantification of transcriptomes, and the genome-wide profiling of DNA-protein interactions. With decreasing cost of NGS, such revolutionary advances in technology has become a powerful tool for studying bacterial genome complexity, which in turn will be used to design synthetic genome. This review describes the NGS approaches, the challenges associated with their application and the advances made so far in characterizing bacterial genomes, transcriptomes, and interactomes. We anticipate these high-throughput data to be a resourceful and broadly used for elucidating bacterial cells at the system level and furthermore, for the synthesis of intelligent biological systems for biotechnological purposes.

Keywords

genome transcriptome interactome nextgeneration sequencing systems biology synthetic biology 

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Jungkon Kim
    • 1
    • 2
  • Sooin Lee
    • 1
  • HyeonSeok Shin
    • 1
    • 2
  • Sun Chang Kim
    • 1
    • 2
    • 3
  • Byung-Kwan Cho
    • 1
    • 2
    • 3
  1. 1.Department of Biological SciencesKorea Advanced Institute of Science and TechnologyDaejeonKorea
  2. 2.Institute for the BioCenturyKorea Advanced Institute of Science and TechnologyDaejeonKorea
  3. 3.Intelligent Synthetic Biology CenterDaejeonKorea

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