Skip to main content
SpringerLink
Log in

Bioconversion of isoflavones during the fermentation of Samso-Eum with Lactobacillus strains

  • Research Paper
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

In this study, the possible application of Lactobacillus strains as a functional starter culture to ferment Samso-Eum (SE), an oriental herbal medicine formula, and the production of bioactive isoflavones (daidzein, genistein) were investigated. Four strains of Lactobacillus (Lactobacillus plantarum KFRI 144, L. amylophilus KFRI 161, L. curvatus KFRI 166, and L. bulgaricus KFRI 344) were used for SE fermentation. Declines in pH and in viable cell counts during fermentation were investigated and the quantification of isoflavones using HPLC were performed after fermentation at 37°C for 48 h. All the tested Lactobacillus strains lowered the pH level to approximately 3.6 after 48 h and showed the highest level of growth at 24 h during SE fermentation. During the SE fermentation of the four Lactobacillus strains, the conversion of isoflavone glycosides (daidzin, genistin) into bioactive aglycones (daidzein, genistein) was observed in all of the fermentations, but with different rates depending on the strains. L. plantarum KFRI 144 and L. amylophilus KFRI 161 exhibited the highest bioconversion rate of isoflavone glycosides into their bioactive aglycones. These results demonstrate that L. plantarum KFRI 144 and L. amylophilus KFRI 161 have potentials as functional starter cultures for manufacturing fermented SE with higher isoflavone bioavailability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Park, B. M., J. E. Hong, Y. C. Ko, J. Y. Shin, and S. H. Lee (2002) The effects of Samsoeum extracts on regional cerebral blood flow and blood pressure in mice. Kor. J. Orient. Int. Med. 23: 25–40.

    Google Scholar 

  2. Ryu, N. H., S. M. Park, H. Kang, B. S. Shim, S. H. Kim, S. H. Choi, and K. S. Ahn (2008) Effects of Samsoeum on cytokine regulation of mouse T cell. Kor. J. Ori. Physiol. Pathol. 22: 856–862.

    Google Scholar 

  3. Kim, H. W., S. J. Cho, B. Y. Kim, J. W. Baek, H. W. Jeong, and S. I. Cho (2007) Effects of Samso-eum on cytokine productions in asthmatic mice. Kor. J. Herbol. 22: 175–180.

    Google Scholar 

  4. Cho, S. J., H. W. Kim, B. Y. Kim, and S. I. Cho (2008) Sam So Eum, a herb extract, as the remedy for allergen-induced asthma in mice. Pulm. Pharmacol. Ther. 21: 578–583.

    Article  CAS  Google Scholar 

  5. Nam, B. S. and Y. B. Kim (2002) The experimental study of Samsoeum and Samsoeumgami-bang on anti-allergic effect. J. Kor. Orient. Med. 15: 343–355.

    Google Scholar 

  6. Lee, S. E., J. Y. Shin, and S. H. Lee (2005) Anti-allergic effects of shensuyin. Kor. J. Orient. Int. Med. 26: 119–128.

    Google Scholar 

  7. Wang, C. Y., H. Y. Huang, K. L. Kuo, and Y. Z. Hsieh (1998) Analysis of Puerariae radix and its medicinal preparations by capillary electrophoresis. J. Chromatogr. A 802: 225–231.

    Article  CAS  Google Scholar 

  8. Zheng, G., D. Yang, D. Wang, F. Zhou, X. Yang, and L. Jiang (2009) Simultaneous determination of five bioactive flavonoids in pericarpium Citri reticulatae from china by high-performance liquid chromatography with dual wavelength detection. J. Agric. Food Chem. 57: 6552–6557.

    Article  CAS  Google Scholar 

  9. Zhang, G. Q., S. G. Ji, Y. F. Chai, Y. T. Wu, and X. P. Yin (1999) Determination of glycyrrhizin in Radix glycyrrhizae and its preparations by capillary zone electrophoresis. Biomed. Chromatogr. 13: 407–409.

    Article  CAS  Google Scholar 

  10. Kim, D. H., K. U. Yu, E. A. Bae, and M. J. Han (1998) Metabolism of puerarin and daidzin by human intestinal bacteria and their relation to in vitro cytotoxicity. Biol. Pharm. Bull. 21: 628–630.

    Article  CAS  Google Scholar 

  11. Murota, K., S. Shimizu, S. Miyamoto, T. Izumi, A. Obata, M. Kikuchi, and J. Terao (2002) Unique uptake and transport of isoflavone aglycones by human intestinal caco-2 cells: Comparison of isoflavonoids and flavonoids. J. Nutr. 132: 1956–1961.

    CAS  Google Scholar 

  12. Masood, M. I., M. I. Qadir, J. H. Shrazi, and I. U. Khan (2011) Beneficial effects of lactic acid bacteria on human beings. Crit. Rev. Microbiol. 37: 91–98.

    Article  Google Scholar 

  13. Michlmayr, H., C. Schumann, N. M. da Silva, K. D. Kulbe, and A. M. del Hierro (2010) Isolation and basic characterization of a beta-glucosidase from a strain of Lactobacillus brevis isolated from a malolactic starter culture. J. Appl. Microbiol. 108: 550–559.

    Article  CAS  Google Scholar 

  14. Cho, Y. H., J. Y. Imm, H. Y. Kim, S. G. Hong, S. J. Hwang, D. J. Park, and S. Oh (2009) Isolation and partial characterization of isoflavone transforming Lactobacillus plantarum YS712 for potential probiotic use. Kor J. Food Sci. Ani. Resour. 29: 640–646.

    Article  Google Scholar 

  15. Eum, H. A., J. H. Lee, M. C. Yang, K. S. Shim, J. H. Lee, and J. Y. Ma (2011) Protective effect of Ssanghwa-Tang fermented by Lactobacillus fermentum against carbon tetrachloride-induced acute hepatotoxicity in rats. Afr. J. Tradit. Complement. Altern. med. 8: 312–321.

    Google Scholar 

  16. Rhee, Y. K., M. H. Kim, Y. C. Lee, J. H. Rho, J. Y. Ma, and C. W. Cho (2011) Characteristics changes of Galgeuntang fermented with lactic acid bacteria. Kor. J. Food Sci. Technol. 43: 655–658.

    Google Scholar 

  17. Hwang, I. K., S. S. Lim, K. H. Choi, K. Y. Yoo, H. K. Shin, E. J. Kim, J. H. Yoon-Park, T. C. Kang, Y. S. Kim, D. Y. Kwon, D. W. Kim, W. K. Moon, and M. H. Won (2006) Neuroprotective effects of roasted licorice, not raw form, on neuronal injury in gerbil hippocampus after transient forebrain ischemia. Acta Pharmacol. Sin. 27: 959–965.

    Article  CAS  Google Scholar 

  18. Pyo, Y. H., T. C. Lee, and Y. C. Lee (2005) Enrichment of bioactive isoflavones in soymilk fermented with β-glucosidase-producing lactic acid bacteria. Food Res. Int. 38: 551–559.

    Article  CAS  Google Scholar 

  19. Donkor, O. N. and N. P. Shah (2008) Production of beta-glucosidase and hydrolysis of isoflavone phytoestrogens by Lactobacillus acidophilus, Bifidobacterium lactis, and Lactobacillus casei in soymilk. J. Food Sci. 73: 15–20.

    Article  Google Scholar 

  20. Chien, H. L., H. Y. Huang, and C. C. Chou (2006) Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria. Food Microbiol. 23: 772–778.

    Article  CAS  Google Scholar 

  21. Mariusz, K. P., J. Yamakoshi, and I. Yukihiko (1999) Daidzein and genistein but not their glucosides are absorbed from the rat stomach. FEBS Lett. 447: 287–291.

    Article  Google Scholar 

  22. Hur, J. W., J. O. Lay, R. D. Beger, J. P. Freeman, and F. Raffi (2000) Isolation of human intestinal bacteria metabolizing the natural isoflavone glycosides, daidzin and genistin. Arch. Microbiol. 174: 422–428.

    Article  CAS  Google Scholar 

  23. Yamakoshi, J., M. K. Piskula, T. Izumi, K. Tobe, M. Saito, S. Kataoka, A. Obata, and M. Kikuchi (2000) Isoflavone aglyconerich extract without soy protein attenuates atherosclerosis development in cholesterol-fed rabbits. J. Nutr. 130: 1695–1699.

    Google Scholar 

  24. Booth, C., D. F. Hargreaves, J. A. Hadfield, A. T. McGown, and C. S. Potten (1999) Isoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro. Br. J. Cancer 80: 1550–1557.

    Article  CAS  Google Scholar 

  25. Nakamura, K., T. Nishihata, J. S. Jin, C. M. Ma, K. Komatsu, M. Iwashima, and M. Hattori (2011) The C-glucosyl bond of puerarin was cleaved hydrolytically by a human intestinal bacterium strain PUE to yield its aglycone daidzein and an intact glucose. Chem. Pharm. Bull. 59: 23–27.

    Article  CAS  Google Scholar 

  26. Kim, D. H. (2000) Herbal Medicinal Microbiology. pp. 147–150. Hyoil Books, Seoul, Korea.

    Google Scholar 

  27. Park, E. K., J. Shin, E. A. Bae, Y. C. Lee, and D. H. Kim (2006) Intestinal bacteria activates estrogenic effect of main constituents puerarin and daidzin of Pueraria thunbergiana. Biol. Pharm. Bull. 29: 2432–2435.

    Article  CAS  Google Scholar 

  28. Rojas, N. L., C. E. Voget, R. A. Hours, and S. F Cavalitto (2011) Purification and characterization of a novel alkaline α-L-rhamnosidase produced by Acrostalangmus luteo albus. J. Ind. Microbiol. Biotechnol. 38: 1515–1522.

    Article  CAS  Google Scholar 

  29. Chung, D. M., Y. C. Chung, P. J. Maeng, and H. K. Chun (2011) Regioselective deglycosylation of onion quercetin glucosides by Saccharomyces cerevisiae. Biotechnol. Lett. 33: 783–786.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Processing Technology Research Group, Korea Food Research Institute, Seongnam, 463-746, Korea

    Chang-Won Cho, Hyun-Cheol Jeong, Hee-Do Hong, Young-Chan Kim, Sang Yoon Choi, Kyungtack Kim & Young-Chul Lee

  2. TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon, 305-811, Korea

    Jin Yeul Ma

Authors
  1. Chang-Won Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyun-Cheol Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee-Do Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Young-Chan Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sang Yoon Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kyungtack Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jin Yeul Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Young-Chul Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jin Yeul Ma or Young-Chul Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cho, CW., Jeong, HC., Hong, HD. et al. Bioconversion of isoflavones during the fermentation of Samso-Eum with Lactobacillus strains. Biotechnol Bioproc E 17, 1062–1067 (2012). https://doi.org/10.1007/s12257-012-0073-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-012-0073-7

Keywords

Search

Navigation