Antioxidant activity of the halophyte Limonium tetragonum and its major active components

  • Jung Im Lee
  • Chang-Suk Kong
  • Myoung Eun Jung
  • Joo Wan Hong
  • Sun Young Lim
  • Youngwan Seo
Research Paper
First Online:
Received:
Revised:
Accepted:

Abstract

In this study, the antioxidant potentials of crude extracts and solvent-partitioned fractions of Limonium tetragonum were assessed by measuring their ability to scavenge intracellular reactive oxygen species (ROS) generated in HT-1080 cells. Following activity-oriented separation, four flavonol glycosides were isolated as active principles and their chemical structures were determined by 2 D NMR and by comparison with reported spectral data. The isolated compounds (1–4) were evaluated for their antioxidant capacity using three different activity tests; degree of occurrence of intracellular ROS, lipid peroxidation in HT-1080 cells and the extent of oxidative damage of genomic DNA purified from HT-1080 cells. All compounds exhibited significantly inhibited the generation of intracellular ROS and lipid peroxidation in HT-1080 cells, and significantly inhibited DNA oxidation. In addition, direct free radical scavenging effects of these compounds were investigated using the electron spin resonance (ESR) spin-trap technique.

Keywords

Limonium tetragonum antioxidant activity reactive oxygen species ESR 
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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Jung Im Lee
    • 1
  • Chang-Suk Kong
    • 2
  • Myoung Eun Jung
    • 1
  • Joo Wan Hong
    • 1
  • Sun Young Lim
    • 1
  • Youngwan Seo
    • 1
  1. 1.Division of Marine Environment and BioscienceKorea Maritime UniversityBusanKorea
  2. 2.Department of Food and NutritionSilla UniversityBusanKorea

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