Biotechnology and Bioprocess Engineering

, Volume 15, Issue 6, pp 905–910 | Cite as

Economic evaluation of off-gas recycle pressure swing adsorption (PSA) in industrial scale poly(3-hydroxybutyrate) fermentation

  • Ho Nam Chang
  • Moon Il Kim
  • Qiang Fei
  • Jin-Dal-Rae Choi
  • Longan Shang
  • Nag-Jong Kim
  • Jung Ae Kim
  • Hyun Gyu Park
Research Paper


The use of high purity oxygen as a feeding gas in microbial fermentation has recent gained huge attention in industrial scale process due to its advantageous effect to increase viable cell density as well as target metabolite productivity. However, usage of highly pure oxygen is currently too expensive for industrialization. To overcome this limitation, off-gas recycle pressure swing adsorption (PSA) was employed which assist in continuous production of high purity oxygen during fermentation process. The off-gas from fermentation process contained about 70 ∼ 80% oxygen which was re-utilized as the input gas in PSA, whereas conventional PSA process utilized air with 21% oxygen content. This difference in the oxygen content of the input gas decreased the size of PSA, resulting in reduction of equipment costs. Using the characteristics of off-gas recycle PSA, we analyzed and compared the economic feasibility of a highly pure oxygen supplying strategy for the production of poly(3-hydroxybutyrate) (PHB) by Ralstonia eutropha based on previously reported data, using both air and highly pure oxygen. To achieve annual production of 3,630 tonnes of PHB the aforementioned method was adopted, by doing so the production cost of PHB was significantly reduced to 4.2 $/kg. While, the conventional process (utilizing air as the feeding gas) resulted in the highest cost (5.9 $/kg). The low cost of off-gas recycle PSA was due to reduction of fixed capital cost, which was about 3 times lower than that of the conventional fermentation process. Therefore, the production cost of fermentation was considerably decreased by using a highly pure oxygen supplying strategy based on off-gas recycle PSA.


fermentation off-gas recycle PSA high purity oxygen economic evaluation 


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  1. 1.
    Shang, L., M. Jiang, C. H. Ryu, H. N. Chang, S. H. Cho, and J. W. Lee (2003) Inhibitory effect of carbon dioxide on the fedbatch culture of Ralstonia eutropha: Evaluation by CO2 pulse injection and autogenous CO2 methods. Biotechnol. Bioeng. 83: 312–320.CrossRefGoogle Scholar
  2. 2.
    Shang, L., P. Y. Tian, N. J. Kim, H. N. Chang, and M. S. Hahm (2009) Effects of oxygen supply modes on the production of human growth hormone in different scale bioreactors. Chem. Eng. Technol. 32: 600–605.CrossRefGoogle Scholar
  3. 3.
    Riesenberg, D. and R. Guthke (1999) High-cell density cultivation of microorganisms. Appl. Microbiol. Biotechnol. 51: 422–430.CrossRefGoogle Scholar
  4. 4.
    Ryu, H. W., S. K. Hahn, Y. K. Chang, and H. N. Chang (1997) Production of poly(3-hydroxybutyric acid) by high cell density fed-batch culture of Alcaligenes eutrophus with phosphate limitation. Biotechnol. Bioeng. 55: 28–32.CrossRefGoogle Scholar
  5. 5.
    Han, S. J., H. N. Chang, and J. Lee (1998) Fed-batch cultivation of an oxygen-dependent inducible promoter system, the nar promoter in Escherichia coli with an inactivated nar operon. Biotechnol. Bioeng. 59: 400–406.CrossRefGoogle Scholar
  6. 6.
    Han, S. J., H. N. Chang, J. A. DeMoss, E. J. Suh, and J. Lee (2000) Development and characterization of an oxygen-dependent inducible promoter system, the modified nar promoter in a mutant Escherichia coli. Biotechnol. Bioeng. 68: 115–120.CrossRefGoogle Scholar
  7. 7.
    Han, S. J., H. N. Chang, and J. Lee (2001) Characterization of an oxygen-dependent inducible promoter, the nar promoter of Escherichia coli, to utilize in metabolic engineering. Biotechnol. Bioeng. 72: 573–576.CrossRefGoogle Scholar
  8. 8.
    Chang, H. N., S. J. Han, S. C. Yim, M. R. Han, and J. Lee (2002) Fed-batch cultures of Escherichia coli cells with oxygendependent nar promoter systems. Adv. Biochem. Eng. Biotechnol. 74: 171–181.Google Scholar
  9. 9.
    Han, M. R., L. Shang, H. N. Chang, S. J. Han, Y. C. Kim, and J. Lee (2006) Fermentation characteristics of a low-oxygen inducible hmp promoter system in Bacillus subtilis LAB1886. J. Chem. Technol. Biotecnol. 81: 1071–1074.CrossRefGoogle Scholar
  10. 10.
    Liu, Y. and J. H. Tay (2001) Strategy for minimization of excess sludge production from the activated sludge process. Biotechnol. Adv. 19: 97–107.CrossRefGoogle Scholar
  11. 11.
    Pinheiro, R., I. Bero, and M. Mota (2003) Growth and β-galactosidase activity in cultures of Kluyveromyces marxianus under increased air pressure. Lett. Appl. Microbiol. 37: 438–442.CrossRefGoogle Scholar
  12. 12.
    Yang, J. D. and N. S. Wang (1992) Oxygen mass transfer enhancement via fermentor headspace pressurization. Biotechnol. Prog. 8: 244–251.CrossRefGoogle Scholar
  13. 13.
    Pinheiro, R., I. Bero, and M. Mota (1997) Physiological behavior of Saccharomyces cerevisiae under increased air and oxygen pressures. Biotechnol. Lett. 19: 703–708.CrossRefGoogle Scholar
  14. 14.
    Bero, I. and M. Mota (1998) Batch and fed-batch cultures of E. coli TB1 at different oxygen transfer rates. Bioproc. Biosyst. Eng. 18: 451–455.Google Scholar
  15. 15.
    Chang, H. N. and J. H. Seo (2001) Biochemical Engineering 2nd edition, pp. 316, Akademi Press (ISSN 89-7616-225-0), Seoul, Korea.Google Scholar
  16. 16.
    Gas Separation Systems.
  17. 17.
    Giddey, S., F. T. Ciacchi, and S. P. S. Badwal (2010) High purity oxygen production with a polymer electrolyte membrane electrolyser. J. Membrane Sci. 346: 227–232.CrossRefGoogle Scholar
  18. 18.
    He, Y., X. Zhu, Q. Li, and W. Yang (2009) Perovskite oxide absorbents for oxygen separation. AIChE J. 55: 3125–3133.CrossRefGoogle Scholar
  19. 19.
    Choi, J. I. and S. Y. Lee (1997) Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation. Bioproc. Biosyst. Eng. 17: 335–342.Google Scholar
  20. 20.
    Choi, J. I. and S. Y. Lee (2000) Economic considerations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacterial fermentation. Appl. Microbiol. Biotechnol. 53: 646–649.CrossRefGoogle Scholar
  21. 21.
    Chang, H. N., B. J. Kim, C. M. Jeong, J. W. Kang, and J. K. Park (2008) Multi-stage CSTR bioreactor system equipped with cell recycle unit. PCT WO 2008/091113 A1 (USA, pending; Korean patent 10-0834110)Google Scholar
  22. 22.
    Byrom, D. (1987) Polymer synthesis by microorganisms: Technology and economics. Trends Biotechnol. 5: 246–250.CrossRefGoogle Scholar
  23. 23.
    Lee, S. Y. (1996) Plastic bacteria? Progress and prospects for poly-hydroxyalkanoate production in bacteria. Trends Biotechnol. 14: 431–438.CrossRefGoogle Scholar
  24. 24.
    Witholt, B. and B. Kessler (1999) Perspectives of medium chain length poly(hydroxyalkanoates), a versatile set of bacterial bioplastics. Curr. Opin. Biotechnol. 10: 279–285.CrossRefGoogle Scholar

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Ho Nam Chang
    • 1
  • Moon Il Kim
    • 1
  • Qiang Fei
    • 1
  • Jin-Dal-Rae Choi
    • 1
  • Longan Shang
    • 1
  • Nag-Jong Kim
    • 1
  • Jung Ae Kim
    • 1
  • Hyun Gyu Park
    • 1
  1. 1.Department of Chemical and Biomolecular EngineeringKAISTDaejeonKorea

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