Biotechnology and Bioprocess Engineering

, Volume 15, Issue 2, pp 277–284 | Cite as

Statistical optimization of culture media for growth and lipid production of Botryococcus braunii LB572

  • Hai-Linh Tran
  • Ji-Sue Kwon
  • Z-Hun Kim
  • Youkwan Oh
  • Choul-Gyun LeeEmail author
Research Paper


Botryococcus braunii has an outstanding ability to produce lipid; however, it is a slow-growing green microalgae. Statistical optimization of growth media was performed to faster growth and to increase lipid concentration. The effect of media composition on the growth of B. braunii LB572 was examined using fractional factorial design and central composite design. The media components examined include sodium carbonate, potassium phosphate, calcium chloride, magnesium sulfate, ferric citrate, and sodium nitrate. The results indicated that potassium phosphate and magnesium sulfate were major impact factors. The optimum concentrations of potassium phosphate and magnesium sulphate were found to be 0.058 and 0.09 g/L, respectively, for growth and 0.083 and 0.1 g/L, respectively, for lipid production. These values were validated using bubble column photobioreactors. Lipid productivity increased to 0.19 g/L/day in lipid-optimized media, with an average biomass productivity of 0.296 g/L/day and 64.96% w/w. In growth-optimized media, lipid productivity was 0.18 g/L/day, with an average biomass productivity of 0.304 g/L/day and 59.56% w/w.


Fractional factorial design central composite design lipid fatty acids microalgae Botryococcus braunii LB572 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Hai-Linh Tran
    • 1
  • Ji-Sue Kwon
    • 1
  • Z-Hun Kim
    • 1
  • Youkwan Oh
    • 2
  • Choul-Gyun Lee
    • 1
    Email author
  1. 1.Institute of Industrial Biotechnology, Department of Biological EngineeringInha UniversityIncheonKorea
  2. 2.Bioenergy Research CenterKorea Institute of Energy ResearchDaejeonKorea

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