Advertisement

Biotechnology and Bioprocess Engineering

, Volume 15, Issue 2, pp 277–284 | Cite as

Statistical optimization of culture media for growth and lipid production of Botryococcus braunii LB572

  • Hai-Linh Tran
  • Ji-Sue Kwon
  • Z-Hun Kim
  • Youkwan Oh
  • Choul-Gyun LeeEmail author
Research Paper

Abstract

Botryococcus braunii has an outstanding ability to produce lipid; however, it is a slow-growing green microalgae. Statistical optimization of growth media was performed to faster growth and to increase lipid concentration. The effect of media composition on the growth of B. braunii LB572 was examined using fractional factorial design and central composite design. The media components examined include sodium carbonate, potassium phosphate, calcium chloride, magnesium sulfate, ferric citrate, and sodium nitrate. The results indicated that potassium phosphate and magnesium sulfate were major impact factors. The optimum concentrations of potassium phosphate and magnesium sulphate were found to be 0.058 and 0.09 g/L, respectively, for growth and 0.083 and 0.1 g/L, respectively, for lipid production. These values were validated using bubble column photobioreactors. Lipid productivity increased to 0.19 g/L/day in lipid-optimized media, with an average biomass productivity of 0.296 g/L/day and 64.96% w/w. In growth-optimized media, lipid productivity was 0.18 g/L/day, with an average biomass productivity of 0.304 g/L/day and 59.56% w/w.

Keywords

Fractional factorial design central composite design lipid fatty acids microalgae Botryococcus braunii LB572 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Miao, X. and Q. Wu (2006) Biodiesel production from heterotrophic microalgal oil. Bioresour. Technol. 97: 841–846.CrossRefGoogle Scholar
  2. 2.
    Chisti, Y. (2007) Biodiesel from microalgae. Biotechnol. Adv. 25: 294–306.CrossRefGoogle Scholar
  3. 3.
    Song, D., J. Fu, and D. Shi (2008) Exploitation of oil-bearing microalgae for biodiesel. Chin. J. Biotechnol. 24: 341–348.CrossRefGoogle Scholar
  4. 4.
    Borowitzka, M. A. (1988) Fats, oils and hydrocarbons. pp. 257–287. In: M. A. Borowitzka and L. J. Borowitzka (eds). Microalgal Biotechnology. Cambridge University Press, Cambridge, London, UK.Google Scholar
  5. 5.
    Banerjee, A., R. Sharma, Y. Chisti, and U. C. Banerjee (2002) Botryococcus braunii: a renewable source of hydrocarbons and other chemicals. Crit. Rev. Biotechnol. 22: 245–279.CrossRefGoogle Scholar
  6. 6.
    Kates, M. (1986) Techniques of lipidology: isolation, analysis and identification of lipids. Laboratory techniques in biochemistry and molecular biology. 2nd ed., pp. 100–111. Elsevier Science Pub. Co., NY, USA.Google Scholar
  7. 7.
    Tran, H. L., S. J. Hong, and C. G. Lee (2009) Evaluation of extraction methods for recovery of fatty acids from Botryococcus braunii LB 572 and Synechocystis sp. PCC 6803. Biotechnol. Bioprocess Eng. 14: 187–192.CrossRefGoogle Scholar
  8. 8.
    Hong, S. J. and C. G. Lee (2008) Statistical optimization of culture media for production of phycobiliprotein by Synechocystis sp. PCC 6701. Biotechnol. Bioprocess Eng. 13: 491–498.CrossRefGoogle Scholar
  9. 9.
    Li, Y., M. Horsman, B. Wang, N. Wu, and C. Lan (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl. Microbiol. Biotechnol. 81: 629–636.CrossRefGoogle Scholar
  10. 10.
    Xiong, W., X. Li, J. Xiang, and Q. Wu (2008) High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Appl. Microbiol. Biotechnol. 78: 29–36.CrossRefGoogle Scholar
  11. 11.
    Metzger, P. and C. Largeau (2005) Botryococcus braunii: a rich source for hydrocarbons and related ether lipids. Appl. Microbiol. Biotechnol. 66: 486–496.CrossRefGoogle Scholar
  12. 12.
    Lee, C. G. (1999) Calculation of light penetration depth in photobioreactors. Biotechnol. Bioprocess Eng. 4: 78–81.CrossRefGoogle Scholar
  13. 13.
    Wackett, L. P. (2008) Biomass to fuels via microbial transformations. Curr. Opin. Chem. Biol. 12: 187–193.CrossRefGoogle Scholar
  14. 14.
    Knothe, G. (2008) “Designer” biodiesel: optimizing fatty ester composition to improve fuel properties. Energy Fuel 22: 1358–1364.CrossRefGoogle Scholar
  15. 15.
    Ahlgren, G., L. Lundstedt, M. Brett, and C. Forsberg (1990) Lipid composition and food quality of some freshwater microalgae. J. Plankton Res. 12: 809–818.CrossRefGoogle Scholar
  16. 16.
    Dayananda, C., R. Sarada, T. R. Shamala, and G. A. Ravishankar (2006) Influence of nitrogen sources on growth, hydrocarbon and fatty acid production by Botryococcus braunii. Asian J. Plant Sci. 5: 799–804.CrossRefGoogle Scholar

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Hai-Linh Tran
    • 1
  • Ji-Sue Kwon
    • 1
  • Z-Hun Kim
    • 1
  • Youkwan Oh
    • 2
  • Choul-Gyun Lee
    • 1
    Email author
  1. 1.Institute of Industrial Biotechnology, Department of Biological EngineeringInha UniversityIncheonKorea
  2. 2.Bioenergy Research CenterKorea Institute of Energy ResearchDaejeonKorea

Personalised recommendations