Biotechnology and Bioprocess Engineering

, Volume 15, Issue 2, pp 299–307 | Cite as

Production of mycelial biomass and exo-polymer by Hericium erinaceus CZ-2: Optimization of nutrients levels using response surface methodology

  • Fengjie Cui
  • Zhiqiang Liu
  • Yin Li
  • Lifeng Ping
  • Liying Ping
  • Zhicai Zhang
  • Lin Lin
  • Ying Dong
  • Daming Huang
Research Paper
First Online:
Received:
Revised:
Accepted:

Abstract

The Doehlert experimental design was used to optimize the production of mycelial biomass and exopolymer from Hericium erinaceus CZ-2 in this study. Statistical analysis showed that the linear and quadric terms of 3 variables: corn flour, yeast extract, and corn steep liquor had significant effects. The optimized combination of these 3 variables was confirmed through validation experiments. The optimal conditions for higher production of mycelial biomass (19.92 g/L) were estimated when the media composition concentrations were set as: 30.85 g/L, corn flour; 2.81 g/L, yeast extract; 16.9 mL/L, corn steep liquor; 10 g/L, glucose; 1 g/L, KH2PO4; and 0.5 g/L, MgSO4·7H2O; while a maximal exo-polymer yield (1.653 g/L) could be achieved when setting concentrations of: 32.71 g/L, corn flour; 2.35 g/L, Yeast extract; 14.42 mL/L, Corn steep liquor; 10 g/L, glucose; 1 g/L, KH2PO4; and 0.5 g/L, MgSO4·7H2O. The upscale production was also investigated using a 15 L fermentor using the optimized medium.

Keywords

Hericium erinaceus doehlert design submerged culture medium optimization biomass exo-polymer 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Barros, L., T. Cruz, P. Baptista, L. M. Estevinho, and I. C. F. R Ferreira (2008) Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food Chem. Toxicol. 46: 2742–2747.CrossRefGoogle Scholar
  2. 2.
    Solomons, G. L. (1975) Submerged culture production of mycelial biomass. Filamentous Fungi 360: 249–264.Google Scholar
  3. 3.
    Fazenda, M. L., R. Seviour, B. McNeil, and L. M. Harvey (2008) Submerged culture fermentation of ‘Higher Fungi’: the macrofungi. Adv. Appl. Microbiol. 63: 33–103.CrossRefGoogle Scholar
  4. 4.
    Fang, Q. H. and J. J. Zhong (2002) Submerged fermentation of higher fungus Ganoderma lucidum for production of valuable bioactive metabolites-ganoderic acid and polysaccharide. Biochem. Eng. J. 10: 61–65.CrossRefGoogle Scholar
  5. 5.
    Cheung, P. C. K. (1996) The hypocholesterolemic effect of extracellular polysaccharide from the submerged fermentation of mushroom. Nutr. Res. 16: 1953–1957.CrossRefGoogle Scholar
  6. 6.
    Kim, S. W., H. J. Hwang, B. C. Lee, and J. W. Yun (2007) Submerged production and characterization of Grifola frondosa polysaccharides — a new application to cosmeceuticals. Food Technol. Biotechnol. 45: 295–305.Google Scholar
  7. 7.
    Cui, F. J., Y. Li, Z. H. Xu, H. Y. Xu, K. Sun, and W. Y. Tao (2006) Optimization of the medium composition for production of mycelial biomass and exo-polymer by Grifola frondosa GF9801 using response surface methodology. Bioresour. Technol. 97: 1209–1216.CrossRefGoogle Scholar
  8. 8.
    Li, Y., Z. Q. Liu, F. J. Cui, Z. S. Liu, and H. Zhao (2007) Application of Plackett-Burman experimental design and Doehlert design to evaluate nutritional requirements for xylanase production by Alternaria mali ND-16. Appl. Microbiol. Biotechnol. 77: 285–291.CrossRefGoogle Scholar
  9. 9.
    Sim, J. H. and A. H. Kamaruddin (2008) Optimization of acetic acid production from synthesis gas by chemolithotrophic bacterium — Clostridium aceticum using statistical approach. Bioresour. Technol. 99: 2724–2735.CrossRefGoogle Scholar
  10. 10.
    Liu, Z. Q., Z. C. Hu, Y. G. Zheng, and Y. C. Shen (2008) Optimization of cultivation conditions for the production of 1,3-dihydroxyacetone by Pichia membranifaciens using response surface methodology. Biochem. Eng. J. 38: 285–291.CrossRefGoogle Scholar
  11. 11.
    Montgomery, D. C. (1997) Design and analysis of experiments. 4th ed., pp. 626–638. John Wiley & Sons, NY, USA.Google Scholar
  12. 12.
    Heo, S. K., H. S. Lee, and S. D. Ha (2009) A predictive model for the growth rate of Bacillus cereus in broth by response surface methodology. Biotechnol. Bioprocess Eng. 14: 202–206.CrossRefGoogle Scholar
  13. 13.
    Stephenie, W., B. M. Kabeir, M. Shuhaimi, M. Rosfarizan, and A. M. Yazid (2007) Growth optimization of a probiotic candidate, bifidobacterium pseudocatenulatum G4, in milk medium using response surface methodology. Biotechnol. Bioprocess Eng. 12: 106–113.CrossRefGoogle Scholar
  14. 14.
    Liu, Z. Q., Y. Li, F. J. Cui, L. F. Ping, J. N. Song, Y. Ravee, L. Q. Jin, Y. P. Xue, J. M. Xu, G. Li, Y. J. Wang, and Y. G. Zheng (2008) Production of octenyl succinic anhydride-modified waxy corn starch and its characterization. J. Agric. Food Chem. 56: 11499–11506.CrossRefGoogle Scholar
  15. 15.
    Ferreira, S. L. C., W. N. L Santos, C. M. Quintella, B. B. Neto, and J. M. Bosque-Sendra (2004) Doehlert matrix: a chemometric tool for analytical chemistry-review. Talanta 63: 1061–1067.CrossRefGoogle Scholar
  16. 16.
    Korn, M. G. A., W. P. C. Santos, M. Korn, and S. L. C. Ferreira (2005) Optimisation of focused-microwave assisted digestion procedure for Kjeldahl nitrogen determination in bean samples by factorial design and Doehlert design. Talanta 65: 710–715.CrossRefGoogle Scholar
  17. 17.
    Tak, V., P. K. Kanaujia, D. Pardasani, R. Kumar, R. K. Srivastava, A. K. Gupta, and D. K. Dubey (2007) Application of Doehlert design in optimizing the determination of degraded products of nerve agents by ion-pair liquid chromatography electrospray ionization tandem mass spectrometry. J. Chromatogr. A. 1161: 198–206.CrossRefGoogle Scholar
  18. 18.
    Taragano, V. M. and A. M. R. Pilosof (1999) Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation. Enzym. Microbial. Tech. 25: 411–419.CrossRefGoogle Scholar
  19. 19.
    Vanot, G., V. Deyris, M. C. Guilhem, R. P. T. Luu, and L. C. Comeau (2001) Optimal design for the maximization of Penicillium cyclopium lipase production. Appl. Microbiol. Biotechnol. 57: 342–345.CrossRefGoogle Scholar
  20. 20.
    Cavalitto, S. F. and C. F. Mignone (2007) Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain. Process Biochem. 42: 175–179.CrossRefGoogle Scholar
  21. 21.
    Imandi, S. B., V. R. Bandaru, S. R. Somalanka, and H. R. Garapati (2007) Optimization of medium constituents for the production of citric acid from byproduct glycerol using Doehlert experimental design. Enzym. Microbial. Tech. 40: 1367–1372.CrossRefGoogle Scholar
  22. 22.
    Ko, H. G., H. G. Park, S. H. Park, C. W. Choi, S. H. Kim, and W. M. Park (2005) Comparative study of mycelial growth and basidiomata formation in seven different species of the edible mushroom genus Hericium. Bioresour. Technol. 96: 1439–1444.CrossRefGoogle Scholar
  23. 23.
    Nagai, K., A. Chiba, T Nishino, T. Kubota, and H. Kawagishi (2006) Dilinoleoyl-phosphatidylethanolamine from Hericium erinaceum protects against ER stress-dependent Neuron cell death via protein kinase C pathway. J. Nutr. Biochem. 17: 525–530.CrossRefGoogle Scholar
  24. 24.
    Mizuno, T., H. Saito, T. Nishitoba, and H. Kawagishi (1995) Antitumor-active substances from mushrooms. Food Rev. Int. 11: 23–61.CrossRefGoogle Scholar
  25. 25.
    Kawagishi, H., M. Ando, H. Sakamoto, S. Yoshida, F. Ojima, Y. Ishiguro, N. Ukai, and S. Furukawa (1991) Hericenones C, D, and E stimulators of nerve growth factor (NGF)-synthesis, from the mushroom Hericium erinaceum. Tetrahedron Lett. 32: 4561–4564.CrossRefGoogle Scholar
  26. 26.
    Yang, B. K., J. B. Park, and C. H. Song (2003) Hypolipidemic effect of an exo-biopolymer produced from a submerged mycelial culture of Hericium erinaceus. Biosci. Biotechnol. Biochem. 67: 1292–1298.CrossRefGoogle Scholar
  27. 27.
    Huang, D. M., F. J. Cui, Y. Li, Z. C. Zhang, J. W. Zhao, X. M. Han, X. Xiao, J. Y. Qian, Q. F. Wu, and G. Q. Guan (2007) Nutritional requirements for the mycelial biomass and exopolymer production by Hericium erinaceus CZ-2. Food Technol. Biotechnol. 45: 389–395.Google Scholar
  28. 28.
    Cui, F. J., W. Y. Tao, Z. H. Xu, W. J. Guo, H. Y. Xu, Z. H. Ao, J. Jin, and Y. Q. Wei (2007) Structural analysis of anti-tumor heteropolysaccharide GFPS1b from the cultured mycelia of Grifola frondosa GF9801. Bioresour. Technol. 98: 395–401.CrossRefGoogle Scholar
  29. 29.
    Doehlert, D. H. (1970) Uniform shell designs. Appl. Stat. 19: 231–239.CrossRefGoogle Scholar
  30. 30.
    Pacheco-Sánchez, M., Y. Boutin, P. Angers, A. Gosselin, and R. J. Tweddell (2007) Inhibitory effect of CDP, a polysaccharide extracted from the mushroom Collybia dryophila, on nitric oxide synthase expression and nitric oxide production in macrophages. Eur. J. Pharmacol. 555: 61–66.CrossRefGoogle Scholar
  31. 31.
    Kitzberger, C. S. G., A. S. Jr, R. C. Pedrosa, and S. R. Salvador Ferreira (2007) Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids. J. Food Engineering 80: 631–638.CrossRefGoogle Scholar
  32. 32.
    Lee, K. B. (2005) Comparison of fermentative capacities of lactobacilli in single and mixed culture in industrial media. Process Biochem. 40: 1559–1564.CrossRefGoogle Scholar

Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Fengjie Cui
    • 1
    • 2
  • Zhiqiang Liu
    • 3
    • 4
  • Yin Li
    • 5
  • Lifeng Ping
    • 6
  • Liying Ping
    • 3
  • Zhicai Zhang
    • 1
  • Lin Lin
    • 1
  • Ying Dong
    • 1
  • Daming Huang
    • 1
  1. 1.School of Food and BiotechnologyJiangsu UniversityZhenjiangChina
  2. 2.Department of Food, Agricultural, and Biological EngineeringThe Ohio State UniversityWoosterUSA
  3. 3.Institute of BioengineeringZhejiang University of TechnologyHangzhouChina
  4. 4.Department of Chemical and Biological Science, NSF-I/UCRC Center for Biocatalysis and Bioprocessing of MacromoleculesPolytechnic Institute of New York UniversityBrooklynUSA
  5. 5.Department of Plant ScienceNorth Dakota State UniversityFargoUSA
  6. 6.Institute of Quality and Standard for Agricultural ProductsZhejiang Academy of Agricultural SciencesHangzhouChina

Personalised recommendations