Biotechnology and Bioprocess Engineering

, Volume 15, Issue 2, pp 246–253

Isolation, purification, and characterization of novel fengycin S from Bacillus amyloliquefaciens LSC04 degrading-crude oil

  • Lee Sang-Cheol
  • Sun-Hee Kim
  • In-Hye Park
  • Soo-Yeol Chung
  • M. Subhosh Chandra
  • Choi Yong-Lark
Research Paper

Abstract

In this study, a biosurfactant-producing bacterial strain was isolated from oil-contaminated soil on the basis of its ability to degrade crude oil and tributyrin (C4:0). LSC04 was identified as Bacillus amyloliquefaciens LSC04 via 16S rRNA gene analysis and partial gyrA gene sequence analysis. The biosurfactants were purified and structural analysis results showed that B. amyloliquefaciens LSC04 generated a lipopeptide biosurfactant. Two main ions of 1,086.9 and 1,491.2 were measured via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The m/z 1,491.2 was shown to correspond to the lipopeptide fengycin B, but the m/z 1,086.9 ion did not correspond to any known lipopeptide. As constituents of the peptides and the lipophilic portion of the m/z 1,491.2; 10 amino acids (Ile-Tyr-Gln-Pro-Val-Glu-Ser-Tyr-Orn-Glu); and β-hydroxy-C17 fatty acid were identified via ESI-MS/MS. Structurally, the lipopeptide of a molecular mass of 1,491.2 differed from fengycin B and fengycin A by a substitution of serine for the threonine residue in position 4, and the amino acid residue in position 6 was equal to that of fengycin A. The major compound, which had a molecular mass of 1,491.2 Da was designated “Fengycin S”.

Keywords

biosurfactant Bacillus amyloliquefaciens fengycin lipopeptide 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Lee Sang-Cheol
    • 1
  • Sun-Hee Kim
    • 1
  • In-Hye Park
    • 1
  • Soo-Yeol Chung
    • 2
  • M. Subhosh Chandra
    • 1
  • Choi Yong-Lark
    • 1
  1. 1.Department of Biotechnology, College of Natural Resources and Life ScienceDong-A UniversityBusanKorea
  2. 2.Department of Food ScienceDong-Ju CollegeBusanKorea

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