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Biotechnology and Bioprocess Engineering

, Volume 14, Issue 4, pp 443–449 | Cite as

Polyaniline based catalase biosensor for the detection of hydrogen peroxide and azide

  • Ravindra P. Singh
  • Da-Yeon Kang
  • Byung-Keun Oh
  • Jeong-Woo ChoiEmail author
Articles

Abstract

The CAT/PANi/ITO bioelectrode has been prepared as a catalase biosensor and shows response for monitoring not only of H2O2 but also azide. The sensor exhibited an excellent response to the H2O2 and azide. The linear range of H2O2 was 0.064∼1 mM and for azide 0.125∼4 mM, respectively. Catalase biosensor was based on the principle of the measurements as the decrease in the differentiation of oxygen level, which has been caused by the inhibition of catalase in the bioactive layer of the biosensor by azide. The repeatability experiments were done in triplicate. The logarithm response of the biosensor to H2O2 (r2 = 0.99), as well as, for azide (r2 = 0.90) were reported, respectively. The bioelectrode was characterized by CV and AFM. The proposed biosensor would be applied for the determination of H2O2 and azide in various biological samples.

Keywords

Polyaniline (PANi) catalase (CAT) hydrogen peroxide (H2O2azide biosensor bioelectrode 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Ravindra P. Singh
    • 2
  • Da-Yeon Kang
    • 1
  • Byung-Keun Oh
    • 1
    • 2
  • Jeong-Woo Choi
    • 1
    • 2
    Email author
  1. 1.Department of Chemical and Biomolecular EngineeringSogang UniversitySeoulKorea
  2. 2.Interdisciplinary Program of Integrated BiotechnologySogang UniversitySeoulKorea

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