Improvement of pristinamycin production by genome shuffling and medium optimization for Streptomyces pristinaespiralis
- 145 Downloads
To isolate an improved pristinamycin producing strain of Streptomyces pristinaespiralis, the technique of Genome shuffling was used which resulted in a high-yield recombinant G 3-56 strain. Strain G 3-56 yielded 322 ± 17 mg/L of pristinamycin which was 11.4-fold higher than that of the initial strain and 3.7-fold higher than strain UN-78 which previously had the highest yield of pristinamycin. The genetic characteristics of the recombinant G 3-56 strain was stable as revealed by our subculture experiments. The optimal production medium was determined using the orthogonal matrix method. Under the optimal medium conditions, the maximum yield of pristinamycin was 412 mg/L with about 1.24-fold higher than the original medium.
Keywordsgenome shuffling orthogonal matrix method protoplast fusion pristinamycin Streptomyces pristinaespiralis
Unable to display preview. Download preview PDF.
- 1.Corvini, P. F. X., H. Gautier, E. Rondags, H. Vivier, J. L. Goergen, and P. Germain (2000) Intracellular pH determination of pristinamycin-producing Streptomyces pristinaespiralis by image analysis. Microbiology. 146: 2671–2678.Google Scholar
- 2.Corvini, P. F. X., S. Delaunay, F. Maujean, E. Rondags, H. Vivier, J.-L. Goergen, and P. Germain (2004) Intracellular pH of Streptomyces pristinaespiralis is correlated to the sequential use of carbon sources during the pristinamycins-producing process. Enz. Microbiol. Tech. 34: 101–107.CrossRefGoogle Scholar
- 3.Voelker, F. and S. Altaba (2001) Nitrogen source governs the patterns of growth and pristinamycin production in Streptomyces pristinaespiralis. Microbiology. 147: 2447–2459.Google Scholar
- 4.Leclercq, R. and P. Courvalin (1991) Intrinsic and unusual resistance to macrolide, lincosamide, and streptogramin antibiotics in bacteria. Antimicrob. Agents Chemother. 35: 1273–1276.Google Scholar
- 14.Wang, H., H. Wang, C. Meng, and Y. H. Guo (2007) Study of breeding Saccharomyces cerevisiae with improved temperature and ethanol tolerance by genome shuffling. Weishengwuxue Tongbao 34: 705–708.Google Scholar
- 15.Hopwood D. A., M. J. Bibb, K. F. Chater, T. Kieser, C. J. Bruton, T. H. M. Kieser, D. J. Lydiate, C. P. Smith, J. M. Ward, and H. Schrempf (1985) Genetic Manipulation of Streptomyces: A Laboratory Manual. p. 356. The John Innes Foundation, Norwich, UK.Google Scholar
- 16.Chen, J. M. and L. T. Xu (1991) Analysis of Antibiotic Industry. 2nd ed., p. 31. Chinese Press of Pharmaceutical Science, Beijing, CN.Google Scholar