Biotechnology and Bioprocess Engineering

, Volume 14, Issue 2, pp 175–179 | Cite as

Improvement of pristinamycin production by genome shuffling and medium optimization for Streptomyces pristinaespiralis

  • Bo Xu
  • Zhihua Jin
  • Qingchao Jin
  • Ninghui Li
  • Peilin Cen


To isolate an improved pristinamycin producing strain of Streptomyces pristinaespiralis, the technique of Genome shuffling was used which resulted in a high-yield recombinant G 3-56 strain. Strain G 3-56 yielded 322 ± 17 mg/L of pristinamycin which was 11.4-fold higher than that of the initial strain and 3.7-fold higher than strain UN-78 which previously had the highest yield of pristinamycin. The genetic characteristics of the recombinant G 3-56 strain was stable as revealed by our subculture experiments. The optimal production medium was determined using the orthogonal matrix method. Under the optimal medium conditions, the maximum yield of pristinamycin was 412 mg/L with about 1.24-fold higher than the original medium.


genome shuffling orthogonal matrix method protoplast fusion pristinamycin Streptomyces pristinaespiralis 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg GmbH 2009

Authors and Affiliations

  • Bo Xu
    • 1
    • 2
  • Zhihua Jin
    • 1
    • 2
  • Qingchao Jin
    • 1
  • Ninghui Li
    • 1
  • Peilin Cen
    • 2
  1. 1.Department of Biochemical and Pharmaceutical Engineering, Ningbo Institute of TechnologyZhejiang UniversityNingboChina
  2. 2.Institute of Bioengineering, College of Materials Science and Chemical EngineeringZhejiang UniversityHangzhouChina

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