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Immunohistochemical Staining in the Assessment of Melanoma Tumor Thickness

  • Kambiz Kamyab-Hesary
  • Alireza Ghanadan
  • Kamran Balighi
  • Seyede Faeze Mousavinia
  • Maryam NasimiEmail author
Original Article
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Abstract

Vertical tumor thickness has great influence in the prognosis and staging of melanoma. The aim of this study was determination of the differences between melanoma tumor thickness in conventional hematoxylin and eosin (H&E) and immunohistochemical techniques. Thirty-six biopsy specimens were included in our study. For each sample, four adjacent tissue sections were stained with H&E, in addition S-100, Melan- A and HMB-45 staining was performed on the next serial sections. The mean thickness of tumor invasion was 2.16, 2.38, 2.22 and 2.29 mm in H&E, S-100, HMB45 and Melan-A sections evaluation, respectively. The mean difference of the Breslow thickness between H&E and S-100 and also, between H&E and Melan-A stained slides were statistically significant (p˂0.05) while no difference was found in the tumor thickness of the H&E and HMB45 staining evaluation (p = 0.278). Greater tumor thickness was observed in 25 lesions (69.4%) with S-100, 20 lesions (55.5%) with Melan-A and 17 (47.2%) lesions in HMB-45 rather than H&E staining. Conclusively, it appears that H&E staining cannot prove the actual size of melanoma invasion in some cases and immunohistochemical examination can be a complementary method in this situations. Of the melanoma associated immunomarkers, the combination of S-100 and Melan-A staining may suffice to measure depth of tumor invasion.

Keywords

Melanoma Breslow thickness Immunohistochemical staining 

Notes

Compliance with Ethical Standards

Conflict of Interest

There is no conflict of interest to declare.

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Copyright information

© Arányi Lajos Foundation 2019

Authors and Affiliations

  • Kambiz Kamyab-Hesary
    • 1
  • Alireza Ghanadan
    • 1
  • Kamran Balighi
    • 2
  • Seyede Faeze Mousavinia
    • 3
  • Maryam Nasimi
    • 2
    Email author
  1. 1.Department of Dermatopathology, Razi HospitalTehran University of Medical SciencesTehranIran
  2. 2.Department of Dermatology, Razi HospitalTehran University of Medical SciencesTehranIran
  3. 3.Tehran University of Medical SciencesTehranIran

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