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Correlations between Histological and Array Comparative Genomic Hybridization Characterizations of Wilms Tumor

  • Ming-Ru Chiang
  • Chi-Wen Kuo
  • Wen-Chung Wang
  • Tai-Cheng Hou
  • Chen-Yun Kuo
  • Meng-Yao Lu
  • Yen-Chein LaiEmail author
Original Article
  • 33 Downloads

Abstract

Wilms tumor, or nephroblastoma, is the most common pediatric renal malignancy. Its diagnosis is principally based on histology. Several genetic loci have been shown to be associated with Wilms tumor formation, including WT1, WT2, FWT1, FWT2, CTNNB1, WTX, and TP53. Other loci, such as 1p, 2q, 7p, 9q, 12q, 14q, 16q, 17p, and 22, have also been implicated in the etiology of Wilms tumor. The aim of this study is to elucidate the molecular pathogenesis of this tumor. In the present study, we analyzed the histological appearance and copy number aberrations using array comparative genomic hybridization of six Wilms tumors without somatic mutation in the WT1 gene. Many chromosomal aberrations on array comparative genomic hybridization analysis revealed that the genetics of Wilms tumors are extremely complex. Amplifications and deletions of large DNA fragments were observed in some samples. Amplifications of NDUFV1, ZIC2, SIX1, NR2F2, MIR1469, SOX9, JAG1, MIR6870, and GNAS were found in all six Wilms tumors. Moreover, amplifications of five genes were identified in the Wilms tumors of stromal type and amplifications of at least 10 genes were identified in the Wilms tumors of epithelial type. Our results indicated that amplifications of nine genes are the essential events in the tumorigenesis of Wilms tumor, which may inform its clinical and therapeutic management. In addition, mixed type Wilms tumor may be the heterogeneous group able to be classified using genetic results of epithelial and stromal components based on immunohistochemistry.

Keywords

Wilms tumor Nephroblastoma Array comparative genomic hybridization Molecular pathogenesis 

Notes

Acknowledgements

This research was supported by Research Grant CSMU-JAH-106-02 from Chung Shan Medical University-Jen-Ai Hospital. The authors would like to thank GenePhile Bioscience Laboratory of Ko’s Obstetrics and Gynecology Clinic for help with acquisition of data.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.

Ethics Approval and Consent to Participate

This study was approved by the Institutional Review Board of Chung Shan Medical University Hospital via grant reference CS2-16003.

Consent for Publication

Not applicable.

Supplementary material

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ESM 1 (PDF 83 kb)
12253_2019_601_MOESM2_ESM.docx (25 kb)
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Copyright information

© Arányi Lajos Foundation 2019

Authors and Affiliations

  1. 1.Department of PediatricsJen-Ai HospitalTaichungTaiwan
  2. 2.Department of PharmacyJen-Ai HospitalTaichungTaiwan
  3. 3.School of Pharmacy, College of PharmacyChina Medical UniversityTaichungTaiwan
  4. 4.Basic Medical Education CenterCentral Taiwan University of Science and TechnologyTaichungTaiwan
  5. 5.Department of Health Policy and ManagementChung Shan Medical UniversityTaichungTaiwan
  6. 6.Department of Obstetrics and GynecologyJen-Ai HospitalTaichungTaiwan
  7. 7.Department of PathologyJen-Ai HospitalTaichungTaiwan
  8. 8.Department of PediatricsNational Taiwan University HospitalTaipeiTaiwan
  9. 9.Department of Medical Laboratory and BiotechnologyChung Shan Medical UniversityTaichungTaiwan

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