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MiR-374a Activates Wnt/β-Catenin Signaling to Promote Osteosarcoma Cell Migration by Targeting WIF-1

  • Weichao Li
  • Zengdong Meng
  • Tiannan Zou
  • Gang Wang
  • Yijing Su
  • Shaoping Yao
  • Xianrun Sun
Original Article
  • 3 Downloads

Abstract

MiR-374a was proved to take part in the initiation and development of several cancers. However, the molecular mechanism of miR-374a in osteosarcoma (OS) cells remains unclear. The aim of our research was to investigate the role of miR-374a in OS cells migration and clarify the potential mechanisms. Quantitative real-time PCR (qRT-PCR) and western blot analysis were applied to evaluate the expression of miR-374a and Wnt inhibitory factor-1 (WIF-1). Bioinformatical methods and luciferase reporter assay were carried out to predict and confirm the combination of miR-374a and WIF-1. Transwell and wound healing assays were performed to detect the migration capacity of OS cells. Lithium chloride (LiCl) was used to investigate the role of LiCl-activated Wnt/β-catenin signaling pathway in regulating cell migration. Our studies revealed that miR-374a was up-regulated whereas WIF-1 was down-regulated in OS cells. Besides, WIF-1 was the target of miR-374a by performing luciferase reporter assay. By transfection of miR-374a inhibitor and/or WIF-1 siRNA to OS cells, we found that miR-374a promoted the migration of OS cells. In addition, the inhibition of WIF-1 abolished the miR-374a inhibitor-induced migration suppression of OS cells. LiCl experiment revealed that miR-374a promoted OS cells migration by regulating Wnt/β-catenin signaling. In conclusion, miR-374a promotes OS cells migration by activating Wnt/β-catenin signaling pathway via targeting WIF-1.

Keywords

Osteosarcoma (OS) miR-374a Migration Wnt inhibitory factor-1 (WIF-1) Wnt/β-catenin Signaling pathway 

Notes

Acknowledgements

This work was supported the Development Program of Kunming University of Science and Technology (Grant No.: KKSY201560051), Yunnan Provincial Science and Technology Department-Kunming Medical University Joint Special Project (Grant No.: 2015FB095) and the Basic Research Project of Yunnan Provincial Science and Technology (Grant No.: 2018FB119).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Arányi Lajos Foundation 2018

Authors and Affiliations

  1. 1.Faculty of Medical ScienceKunming University of Science and TechnologyKunmingChina
  2. 2.Department of Orthopedic Surgery, The First People’s Hospital of Yunnan ProvinceAffiliated Hospital of Kunming University of Science and TechnologyKunmingChina

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