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Chicken or the Egg: Microbial Alterations in Biopsy Samples of Patients with Oral Potentially Malignant Disorders

  • Gabor Decsi
  • Jozsef Soki
  • Bernadett Pap
  • Gabriella Dobra
  • Maria Harmati
  • Sandor Kormondi
  • Tibor Pankotai
  • Gabor Braunitzer
  • Janos Minarovits
  • Istvan Sonkodi
  • Edit Urban
  • Istvan Balazs Nemeth
  • Katalin Nagy
  • Krisztina Buzas
Original Article

Abstract

Oral carcinogenesis often leads to the alteration of the microbiota at the site of the tumor, but data are scarce regarding the microbial communities of oral potentially malignant disorders (OPMDs). Punch biopsies were taken from healthy and non-healthy mucosa of OPMD patients to analyze the microbiome using metagenome sequencing. In healthy oral mucosa biopsies the bacterial phyla Firmicutes, Fusobacteria, Proteobacteria, Actinobacteria and Bacteroidetes were detected by Ion Torrent sequencing. The same phyla as well as the phyla Fibrobacteres and Spirochaetes were present in the OPMD biopsies. On the species level, there were 10 bacterial species unique to the healthy tissue and 35 species unique to the OPMD lesions whereas eight species were detected in both samples. We observed that the relative abundance of Streptococcus mitis decreased in the OPMD lesions compared to the uninvolved tissue. In contrast, the relative abundance of Fusobacterium nucleatum, implicated in carcinogenesis, was elevated in OPMD. We detected markedly increased bacterial diversity in the OPMD lesions compared to the healthy oral mucosa. The ratio of S. mitis and F. nucleatum are characteristically altered in the OPMD lesions compared to the healthy mucosa.

Keywords

OPMD Oral microbiome Metagenome sequencing Lichen Leukoplakia 

Notes

Acknowledgements

This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, János Bolyai Research Scholarship of the Hungarian Academy of Sciences and GINOP 2.3.2-15-2016-00011.

Compliance with Ethical Standards

Conflict of Interest

No potential conflict of interest was reported by the authors.

Ethical Approval

The study protocol conformed to the Declaration of Helsinki in all respects and was approved by the Institutional Ethics Committee of the University of Szeged (No. 3161).

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Copyright information

© Arányi Lajos Foundation 2018

Authors and Affiliations

  • Gabor Decsi
    • 1
  • Jozsef Soki
    • 2
  • Bernadett Pap
    • 3
  • Gabriella Dobra
    • 3
  • Maria Harmati
    • 3
  • Sandor Kormondi
    • 4
  • Tibor Pankotai
    • 5
  • Gabor Braunitzer
    • 6
  • Janos Minarovits
    • 7
  • Istvan Sonkodi
    • 1
  • Edit Urban
    • 2
  • Istvan Balazs Nemeth
    • 8
  • Katalin Nagy
    • 1
  • Krisztina Buzas
    • 3
    • 7
  1. 1.Faculty of Dentistry, Department of Oral SurgeryUniversity of SzegedSzegedHungary
  2. 2.Albert Szent-Gyorgyi Clinical Centre, Institute of Clinical MicrobiologyUniversity of SzegedSzegedHungary
  3. 3.Biological Research CentreHungarian Academy of SciencesSzegedHungary
  4. 4.Albert Szent-Gyorgyi Clinical Centre, Department of TraumatologyUniversity of SzegedSzegedHungary
  5. 5.Department of Biochemistry and Molecular BiologyUniversity of SzegedSzegedHungary
  6. 6.dicomLAB Ltd.SzegedHungary
  7. 7.Faculty of Dentistry, Department of Oral Biology and Experimental Dental ResearchUniversity of SzegedSzegedHungary
  8. 8.Department of Dermatology and AllergologyUniversity of SzegedSzegedHungary

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