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Pathology & Oncology Research

, Volume 25, Issue 1, pp 421–427 | Cite as

Association Between Polymorphisms in the Promoter Region of microRNA-34b/c and the Chemoradiotherapy Efficacy for Locally Advanced Esophageal Squamous Cell Carcinoma in Chinese Han Population

  • Jilisihan Bulibu
  • Wei Wang
  • Yong Tang
  • Na Li
  • Keyoumu SaifudingEmail author
Original Article
  • 59 Downloads

Abstract

The study aims to explore the association between polymorphisms in the promoter region of microRNA-34b/c (miR-34b/c) and the chemoradiotherapy efficacy for locally advanced esophageal squamous cell carcinoma (ESCC) in Chinese Han population. A total of 175 locally advanced ESCC cases and 186 healthy individuals were enrolled as the case and control groups. Denaturing high performance liquid chromatography (DHPLC) was applied to determine the genotypes of subjects. Subjects in the case group were classified into complete response (CR), partial response (PR), stable disease (SD) and progressive disease (PD). CR + PR were defined as the sensitive group, and SD + PD were defined as the resistance group. All patients were followed up for 3 ~ 36 months. Receiver operating characteristic (ROC) curve was used to evaluate the predictive value of rs4938723 in the promoter region of miR-34b/c in the chemoradiotherapy efficacy for patients with locally advanced ESCC. The distribution of genotype and allele of rs4938723 in the promoter region of miR-34b/c was significantly different between the case and control group (both P < 0.05), and CC genotype and C allele could decrease the risk of ESCC (CC genotype: OR = 0.57, 95%CI = 0.32 ~ 0.99, P = 0.045; C allele: OR = 0.72, 95%CI = 0.54 ~ 0.97, P = 0.032). MiR-34b/c rs4938723 was associated with ESCC TNM staging, differentiation degree, and lymph node metastasis (LNM) for ES CC patients (all P < 0.05). The chemoradiotherapy efficacy of patients with CC genotype was better than that of patients with (TT + TC) genotypes (P < 0.05). ROC curve results showed that the area under curve (AUC), sensitivity and specificity were 0.777, 85.1% and 71.3%, respectively. The average median progression free survival (PFS) of patients with (TT + TC) genotypes was significantly shorter than those patients with CC genotype (P < 0.05). Our study provides evidence that miR-34b/c rs4938723 is closely related with the chemoradiotherapy efficacy for locally advanced ESCC.

Keywords

MicroRNA-34b/c Esophageal squamous cell carcinoma Promoter region Polymorphism Chemoradiotherapy efficacy Chinese Han population 

Notes

Acknowledgements

We would like to thank all the people for their technical assistance and valuable advice.

Author Contribution

JB and YT designed the study. WW and KS collated the data, designed and developed the database, carried out data analyses and produced the initial draft of the manuscript. NL contributed to drafting the manuscript. All authors have read and approved the final submitted manuscript.

Compliance with Ethical Standards

Conflict of Interest

None.

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Copyright information

© Arányi Lajos Foundation 2017

Authors and Affiliations

  • Jilisihan Bulibu
    • 1
  • Wei Wang
    • 1
  • Yong Tang
    • 1
  • Na Li
    • 1
  • Keyoumu Saifuding
    • 1
    Email author
  1. 1.Department of GastroenterologyThe Affiliated Tumor Hospital of Xinjiang Medical UniversityUrumqiPeople’s Republic of China

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