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Pathology & Oncology Research

, Volume 25, Issue 1, pp 107–114 | Cite as

Genetic Variations of DNA Repair Genes in Breast Cancer

  • Asuman ÖzgözEmail author
  • Kuyaş Hekimler Öztürk
  • Ayşegül Yükseltürk
  • Hale Şamlı
  • Zuhal Başkan
  • Fadime Mutlu İçduygu
  • Mehmet Bacaksız
Original Article

Abstract

Genetic variations in DNA repair genes may affect DNA repair capacity therefore increase risk for cancer. In our study, we evaluted the relation between DNA repair gene polymorphisms XRCC1 rs1799782, rs25487, rs25489; XPC rs2228000, rs2228001; XPD rs1799793, rs13181; XRCC3 rs861539; RAD51B rs10483813, rs1314913 and breast cancer risk for 202 Turkish cases in total, in which 102 patients with breast cancer and 100 controls. Genotyping of the DNA samples was carried out by multiplex PCR and matrix-assisted laser desorption/ionization mass spectrometry with time of flight measurement (MALDI-TOF) using Sequenom MassARRAY 4 analyzer. Genotype and allele distributions were calculated between the groups. Odds ratios (ORs) and 95% confidence intervals (CIs) were reported. rs25487 AA genotype and A allele was found to be increased in the control group (respectively, OR 0.16 95% CI 0.02–1.06, p = 0.058; OR 1.55, 95% CI 1.01–2.36, p = 0.043) and rs861539 T allele was found to be decreased in the patient group (OR 1.53, 95% CI 1.01–2.30, p = 0.049). No association with breast cancer was found for the remaining SNPs. Our findings suggest that XRCC1 rs25487 AA genotype and A allele, XRCC3 rs861539 T allele may have protective effects in breast cancer for Turkish population.

Keywords

Breast cancer DNA damage DNA repair Polymorphism 

Notes

Acknowledgments

This study was supported by the Kastamonu University Scientific Research Projects Coordination Unit with the project number KÜBAP-01/2013-03. We thank very much to Assoc. Prof. Dr. Ergin Murat ALTUNER for language editing of the manuscript.

Compliance with Ethical Standards

Human Blood samples used in the study were obtained using protocols approved by the Acıbadem University Medical Research Assessment Committee. The written informed consent was taken from all the cases participated in the study.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Arányi Lajos Foundation 2017

Authors and Affiliations

  • Asuman Özgöz
    • 1
    Email author
  • Kuyaş Hekimler Öztürk
    • 2
  • Ayşegül Yükseltürk
    • 3
  • Hale Şamlı
    • 4
  • Zuhal Başkan
    • 5
  • Fadime Mutlu İçduygu
    • 6
  • Mehmet Bacaksız
    • 7
  1. 1.Kastamonu School of Medicine, Department of Medical GeneticsKastamonu UniversityKastamonuTurkey
  2. 2.School of Medicine, Department of Medical GeneticsSüleyman Demirel UniversityIspartaTurkey
  3. 3.Fazıl Boyner Faculty of Health Sciences, Department of Nutrition and DieteticsKastamonu UniversityKastamonuTurkey
  4. 4.School of Veterinary Medicine, Department of GeneticsUludag UniversityBursaTurkey
  5. 5.School of Medicine, Department of Medical OncologyAcıbadem UniversityIstanbulTurkey
  6. 6.School of Medicine, Department of Medical GeneticsGiresun UniversityGiresunTurkey
  7. 7.Alanya Çıplaklı Family Health CenterAlanya-AntalyaTurkey

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