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Krüppel-Like Factor 8 Overexpression Correlates with Poor Prognosis in Non-Small Cell Lung Cancer

  • Jianchao Li
  • Yifei Liu
  • Jianhua Xue
  • Mingming Xu
  • Jianguo Zhang
  • Junhua Liu
  • Wenyi Wang
Original Article

Abstract

Krüppel-like factor 8 (KLF8) plays a key role in cancer progression. However, its expression pattern and relationship with clinicopathological characteristics in non-small cell lung cancer (NSCLC) has not been completely elucidated. The study aimed to investigate the expression of KLF8 and its correlation with clinical pathologic features in NSCLC and to explore the potential mechanism. The expression of KLF8 in NSCLC was detected by RT-PCR, Western blot and immunohistochemistry. The expression of Vimentin and E-cadherin, epithelial-mesenchymal transition (EMT) markers, were detected by immunohistochemistry in the NSCLC. The relationship between KLF8 expression and various clinicopathological features or EMT markers was investigated. The results showed m-RNA and protein of KLF8 were overexpressed in NSCLC and the percent of KLF8 positive cells was positively correlated with TNM stage, lymph node metastasis and poor overall survive. Moreover, high expression of KLF8 correlated with E-cadherin low expression, and Vimentin overexpression. Additionally, COX multivariate regression analysis suggested that TNM stage, KLF8, E-cadherin and Vimentin were independent prognostic indicators for overall survival of patients with NSCLC. The data demonstrate that KLF8 is overexpressed in NSCLC. KLF8 overexpression promotes the malignancy of NSCLC, which mechanism may be involved in EMT. KLF8 maybe serve as a potential therapeutic target for NSCLC.

Keywords

Krüppel-like factor 8 Non-small cell lung cancer Pithelial-mesenchymal transition Prognosis 

Notes

Compliance with Ethical Standards

Conflict of Interest

None.

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Copyright information

© Arányi Lajos Foundation 2017

Authors and Affiliations

  1. 1.Department of Thoracic surgery the First People’s Hospital of YanchengYanchengChina
  2. 2.Department of Thoracic surgerythe Affiliated Hospital of Nantong UniversityNantongChina
  3. 3.Department of PathologyThe Affiliated Hospital of Nantong UniversityNantongChina

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