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Pathology & Oncology Research

, Volume 23, Issue 1, pp 125–130 | Cite as

Frameshift Mutations in the Mononucleotide Repeats of TAF1 and TAF1L Genes in Gastric and Colorectal Cancers with Regional Heterogeneity

  • Hye Rim Oh
  • Chang Hyeok An
  • Nam Jin Yoo
  • Sug Hyung LeeEmail author
Original Article

Abstract

Initiation of transcription by RNA polymerase II requires TATA-box-binding protein (TBP)-associated factors (TAFs). TAF1 is a major scaffold by which TBP and TAFs interact in the basal transcription factor. TAF1L is a TAF1 homologue with 95 % amino acid identity with TAF1. TAF1 is involved in apoptosis induction and cell cycle regulation, but roles of TAF1 and TAF1L in tumorigenesis remain unknown. The aim of this study was to explore whether TAF1 and TAF1L genes were mutated in gastric (GC) and colorectal cancers (CRC). In a public database, we found that TAF1 and TAF1L genes had mononucleotide repeats in the coding sequences that might be mutation targets in the cancers with microsatellite instability (MSI). We analyzed the mutations in 79 GC and 124 CRC by single-strand conformation polymorphism analysis and DNA sequencing. In the present study, we found TAF1 frameshift mutations (3.8 % of CRC with MSI-H) and TAF1L frameshift mutations (2.9 % of GC and 3.8 % of CRC with MSI-H). These mutations were not found in stable MSI/low MSI (MSS/MSI-L) (0/90). In addition, we analyzed intratumoral heterogeneity (ITH) of TAF1 and TAF1L frameshift mutations in 16 CRC and found that two and one CRC harbored regional ITH of TAF1 and TAF1L frameshift mutations, respectively. Our data indicate that TAF1 and TAF1L genes harbored not only somatic mutations but also mutational ITH, which together might play a role in tumorigenesis of GC and CRC with MSI-H. Our results also suggest that ultra-regional mutation analysis is required for a comprehensive evaluation of mutation status in these tumors.

Keywords

TAF1 TAF1L Basal transcription component Mutation Cancers Microsatellite instability 

Notes

Acknowledgments

This work was supported grants from National Reserach Foundation of Korea (2012R1A5A2047939 and 2015R1D1A1A01057355).

Compliance with Ethical Standards

Conflict of Interest

The authors declare no competing interests.

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Copyright information

© Arányi Lajos Foundation 2016

Authors and Affiliations

  • Hye Rim Oh
    • 1
  • Chang Hyeok An
    • 2
  • Nam Jin Yoo
    • 1
  • Sug Hyung Lee
    • 1
    Email author
  1. 1.Department of Pathology, College of MedicineThe Catholic University of KoreaSeoulSouth Korea
  2. 2.Department of Surgery, College of MedicineThe Catholic University of KoreaSeoulSouth Korea

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