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Pathology & Oncology Research

, Volume 21, Issue 3, pp 597–604 | Cite as

Expression of Certain Leukemia/Lymphoma Related microRNAs and its Correlation with Prognosis in Childhood Acute Lymphoblastic Leukemia

  • Karolina Nemes
  • Monika CsókaEmail author
  • Noémi Nagy
  • Ágnes Márk
  • Zsófia Váradi
  • Titanilla Dankó
  • Gábor Kovács
  • László Kopper
  • Anna Sebestyén
Research

Abstract

In spite of the improved efficacy of therapy, it still fails in 15–20 % of childhood acute lymphoblastic leukemia (ALL) patients. Recently, altered expression of certain miRNAs (miRs) have been described in ALL with potential effect on prognosis. Presence of certain miRs (miRNA-16, −21, −24, −29b, −128b, −142-3p, −155, −223) was characterized in human lymphoma and leukemia cells by real-time PCR. Expression of miRs in pediatric ALL patients (n = 24) was measured before chemotherapy, at conventional response checkpoints and at relapse. Correlation between altered miR expression and response to prednisolone at day 8 of therapy and long term prognosis was statistically analysed. Overexpression of “oncomiR/inflammamiR”-21 – which is characteristic in different tumors—was missing in human ALL cells. However, higher expression of miR-128b and lower expression of miR-223 is generally characteristic for human ALL cell lines and ALL cells isolated from pediatric patients. Correlation was shown between miR-128b expression and prognosis, prednisolone response and survival data in childhood ALL. Expression of miR-128b and miR-223—both are leukemia specific—changed in parallel with percentage of bone marrow blasts in remission and during relapse. Therefore, we suggest that overexpression of miR-128b and downregulation of miR-223 shows a significant correlation with treatment response and prognosis in childhood ALL.

Keywords

miRNA-128b miRNA-223 Childhood ALL Prognosis Relapse 

Notes

Acknowledgments

We thank Gézáné Csorba, András Weiperth, István Kenessey, Csaba Bödör, Tibor Füle, Hajnalka Rajnai for discussion and technical assistance. CCRF-CEM, Nalm6 and Mn60 ALL cell lines were a kind gift of Edit Buzás and Beáta Scholtz from cell culture laboratories at Semmelweis University, Budapest, and The Medical and Health Science Centre, University of Debrecen. The work was supported by Sebestyén A. and Kopper L. OTKA projects (K68341, K81624, K84262) of the Hungarian Academy of Sciences.

Conflict of interest

We declare that all authors have no financial or other conflict of interest that might bias their work.

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Copyright information

© Arányi Lajos Foundation 2014

Authors and Affiliations

  • Karolina Nemes
    • 1
    • 2
  • Monika Csóka
    • 1
    Email author
  • Noémi Nagy
    • 2
  • Ágnes Márk
    • 2
  • Zsófia Váradi
    • 1
  • Titanilla Dankó
    • 2
  • Gábor Kovács
    • 1
  • László Kopper
    • 2
  • Anna Sebestyén
    • 2
    • 3
  1. 1.2nd Department of PediatricsSemmelweis UniversityBudapestHungary
  2. 2.1st Department of Pathology and Experimental Cancer ResearchSemmelweis UniversityBudapestHungary
  3. 3.Tumor Progression Research Group of Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis UniversityBudapestHungary

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