The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade
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Here, we report the p53 dependent mitochondria-mediated apoptotic mechanism of plant derived silver-nanoparticle (PD-AgNPs) in colorectal cancer cells (CRCs). PD-AgNPs was synthesized by reduction of AgNO3 with leaf extract of a medicinal plant periwinkle and characterized. Uptake of PD-AgNPs (ξ - 2.52 ± 4.31 mV) in HCT116 cells was 3 fold higher in comparison to synthetic AgNPs (ξ +2.293 ± 5.1 mV). A dose dependent increase in ROS production, activated JNK and decreased mitochondrial membrane potential (MMP) were noted in HCT116 but not in HCT116 p53 −/− cells after PD-AgNP exposure. PD-AgNP-mediated apoptosis in CRCs is a p53 dependent process involving ROS and JNK cascade.
KeywordsSilver nanoparticle Periwinkle p53 MAPK MMP ROS Colon cancer
Authors are greatly acknowledged Prof. Bert Vogelstein, Johns Hopkins, Baltimore, USA for providing HCT116 p53 −/− cell lines and also thanks to ICMR and DBT, Govt. of India for financial support.
Conflict of Interest
The authors declare no conflict of interest.
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