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Pathology & Oncology Research

, Volume 21, Issue 2, pp 333–338 | Cite as

Apoptotic Effects of Quercitrin on DLD-1 Colon Cancer Cell Line

  • Zeynep Birsu Cincin
  • Miray Unlu
  • Bayram Kiran
  • Elif Sinem Bireller
  • Yusuf Baran
  • Bedia CakmakogluEmail author
Research

Abstract

Quercetin, which is the most abundant bioflavonoid compound, is mainly present in the glycoside form of quercitrin. Although different studies indicated that quercitrin is a potent antioxidant, the action of this compound is not well understood. In this study, we investigated whether quercitrin has apoptotic and antiproliferative effects in DLD-1 colon cancer cell lines. Time and dose dependent antiproliferative and apoptotic effects of quercitrin were subsequently determined by WST-1 cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, detection of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP) and also the localization of phosphatidylserine (PS) in the plasma membrane. There were significant increases in caspase-3 activity, loss of MMP, and increases in the apoptotic cell population in response to quercitrin in DLD-1 colon cancer cells in a time- and dose-dependent manner. These results revealed that quercitrin has antiproliferative and apoptotic effects on colon cancer cells. Quercitrin activity supported with in vivo analyses could be a biomarker candicate for early colorectal carcinoma.

Keywords

Colon Cancer Quercitrin Antiproliferative Apoptosis 

Notes

Acknowledgments

This work was supported by the Istanbul University Scientific Research Projects; 31162. We would like to thank to Mr. David Chapman for English editing.

Conflict of interest

The authors declare that they have no financial disclosures or conflicts of interest.

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Copyright information

© Arányi Lajos Foundation 2014

Authors and Affiliations

  • Zeynep Birsu Cincin
    • 1
  • Miray Unlu
    • 2
  • Bayram Kiran
    • 3
  • Elif Sinem Bireller
    • 1
  • Yusuf Baran
    • 2
  • Bedia Cakmakoglu
    • 1
    Email author
  1. 1.Istanbul University, Institute of Experimental Medical ResearchDepartment of Molecular MedicineCapaTurkey
  2. 2.Izmir Institute of Technology, Science FacultyDepartment of Molecular Biology and GeneticsUrlaTurkey
  3. 3.Kastamonu UniversityDepartment of BiologyKastamonuTurkey

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