Cyclooxygenase-2 Polymorphisms and Breast Cancer Associated Risk in Pakistani Patients
- 143 Downloads
Prostaglandins produced by Cyclooxygenase-2 enzyme have been implicated to have a role in breast carcinogenesis. Several single nucleotide polymorphisms (SNPs) linked to COX-2 enzyme are reported to modulate its expression. The aim of the present study was to examine association of these SNPs to breast cancer risk in Pakistani patients. Methods In this case–control study, three sequence variants rs689465, rs689466, rs20417 in the promoter region of COX-2 were screened to evaluate the association with breast cancer risk. A total of 150 breast cancer patients and 101 healthy control genomic DNA were genotyped for rs689456, rs689466, rs20417 and their genotypes distribution in cases and control were compared using Pearson chi square test. Risk association was analyzed through odd ratio calculated by logistic regression. Results A screening analysis of COX-2 SNPs in 101 healthy controls showed distribution of Minor allelic frequency distribution of SNPs as follows : rs689465 (0.12), rs689466 (0.15), rs20417 (0.23). Further analyses revealed that their observed genotype frequencies were consistent with Hardy Weinberg equilibrium and strong linkage disequilibrium was identified between rs20417, rs689465 and rs689466. The Combined allele variants analysis showed that Haplotype rs68965G- 689466A-20417C (OR 2.909; CI 95 %1.3776.327; P = 0.007) was significantly associated with breast cancer. Conclusions Our results indicate no strong association between three most frequent COX-2 SNPs rs689465 rs689466, rs20417 studied with breast cancer risk in the single locus analysis. However, our data suggested that combined COX-2 SNP haplotype have a role in breast cancer associated risk in Pakistani patients.
KeywordsCyclooxygenase Cox-2 Breast Cancer Polymorphism Haplotypes RFLP
- 9.Hu HM, Kuo CH, Lee CH, Wu IC, Lee KW, Lee JM, et al (2009) Polymorphism in COX-2 modifies the inverse association between Helicobacter pylori seropositivity and esophageal squamous cell carcinoma risk in Taiwan: a case control study. BMC Gastroenterol 9:37Google Scholar
- 15.Zang X, Miao X, Tan W, Ning B, Liu Z, Hong Y (2005) Identification of functional genetic variants in cyclooxygenase-2 and their association with risk of oseophageal cancer. Gastroenterolgy 129:565–576Google Scholar
- 22.Ahmed F (1996) Pakistan: Ethnic fragmentation or national integration. Pak Dev Rev 35:631–645Google Scholar
- 26.Brasky TM, Bonner MR, Moysich KB, Ambrosone CB, Nie J, Tao MH et al (2011) Genetic variant in Cox-2, Non-steroidal anti-inflammatory drug and breast cancer risk in the Western New York Exposures and Breast Cancer (WEB) Study. Breast Cancer Res Treat 126:157–165PubMedCentralPubMedCrossRefGoogle Scholar