Characterization of the Attenuation of Breast Cancer Bone Metastasis in Mice by Zoledronic Acid Using 99mTc bone Scintigraphy
- 270 Downloads
Metastatic breast cancer often metastasizes to bone. The purposes of the study were (1) to evaluate the use of 99mTc-MDP bone scintigraphy for detection of metastatic bone lesions, and (2) to determine the efficacy of zoledronic acid in mice with breast cancer bone metastasis. All tumor-bearing mice were analyzed with radionuclide bone scintigraphy, X-ray, and histological analysis. The metastatic bone tissue was also harvested and analyzed by western blotting and real-time qPCR. Interestingly, zoledronic acid significantly decreased both the tumor burden and the incidence of bone metastasis in mice. In addition, histomorphometric, stereological, and molecular biology analyses demonstrated that zoledronic acid may function to inhibit breast cancer cell growth in the bone microenvironment and regulate the function of osteoblasts and osteoclasts in tumor-bearing mice. Finally, the attenuation of breast cancer bone metastasis using zoledronic acid can be accurately characterized by 99mTc bone scintigraphy in mice.
KeywordsBone metastasis Breast cancer 99mTc-MDP bone scintigraphy Zoleronic acid
This research was supported by The National Natural Science Foundation of China (No. 30772811, No. 81102597 and No. 81173269), “Shu Guang” project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation (No. 08GG12) and Sponsored by Program of Shanghai Subject Chief Scientist (No. 10XD1404000).
Conflict of Interest
We have no conflict of interest to declare.
- 3.Lipton A, Uzzo R, Amato RJ, Ellis GK, Hakimian B, Roodman GD et al (2009) The science and practice of bone health in oncology: managing bone loss and metastasis in patients with solid tumors. J Natl Compr Cancer Netw Suppl 7:S1–S29Google Scholar
- 5.Anderson KC, Alsina M, Bensinger W, Biermann JS, Chanan-Khan A, Cohen AD, et al (2010) NCCN clinical practice guidelines in oncology: multiple myeloma, 2010, v.2. Available at: http://www.24hmb.com/UpLoad/Editor/2010/1/4/2010010471312017.pdf
- 17.Padhani A, Husband J (1998) Bone metastases. In: Husband JES, Reznek RH (eds) Imaging in oncology oxford. Isis Medical Media Ltd, U.K., pp 765–787Google Scholar