Pathology & Oncology Research

, Volume 20, Issue 3, pp 747–754 | Cite as

Characterization of the Attenuation of Breast Cancer Bone Metastasis in Mice by Zoledronic Acid Using 99mTc bone Scintigraphy

  • Chunyu Wu
  • Shunfang Yang
  • Zhenping Sun
  • Xianghui Han
  • Yiyi Ye
  • Sheng Liu
Research

Abstract

Metastatic breast cancer often metastasizes to bone. The purposes of the study were (1) to evaluate the use of 99mTc-MDP bone scintigraphy for detection of metastatic bone lesions, and (2) to determine the efficacy of zoledronic acid in mice with breast cancer bone metastasis. All tumor-bearing mice were analyzed with radionuclide bone scintigraphy, X-ray, and histological analysis. The metastatic bone tissue was also harvested and analyzed by western blotting and real-time qPCR. Interestingly, zoledronic acid significantly decreased both the tumor burden and the incidence of bone metastasis in mice. In addition, histomorphometric, stereological, and molecular biology analyses demonstrated that zoledronic acid may function to inhibit breast cancer cell growth in the bone microenvironment and regulate the function of osteoblasts and osteoclasts in tumor-bearing mice. Finally, the attenuation of breast cancer bone metastasis using zoledronic acid can be accurately characterized by 99mTc bone scintigraphy in mice.

Keywords

Bone metastasis Breast cancer 99mTc-MDP bone scintigraphy Zoleronic acid 

Notes

Acknowledgments

This research was supported by The National Natural Science Foundation of China (No. 30772811, No. 81102597 and No. 81173269), “Shu Guang” project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation (No. 08GG12) and Sponsored by Program of Shanghai Subject Chief Scientist (No. 10XD1404000).

Conflict of Interest

We have no conflict of interest to declare.

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Copyright information

© Arányi Lajos Foundation 2014

Authors and Affiliations

  • Chunyu Wu
    • 1
  • Shunfang Yang
    • 2
  • Zhenping Sun
    • 1
  • Xianghui Han
    • 3
  • Yiyi Ye
    • 3
  • Sheng Liu
    • 1
    • 3
    • 4
  1. 1.Department of Breast Surgery, Longhua HospitalShanghai University of Traditional Chinese MedicineShanghaiChina
  2. 2.Department of Nuclear Medicine, Shanghai Chest HospitalShanghai JiaoTong UniversityShanghaiChina
  3. 3.Pharmacology Laboratory of Traditional Chinese Medicine, Longhua HospitalShanghai University of Traditional Chinese MedicineShanghaiChina
  4. 4.Department of Breast Surgery and Pharmacology Laboratory of Traditional Chinese Medicine, Long Hua HospitalShanghai University of Traditional Chinese MedicineShanghaiChina

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