Pathology & Oncology Research

, Volume 19, Issue 4, pp 785–794 | Cite as

Tumor-Related Gene Changes in Immunosuppressive Syrian Hamster Cholangiocarcinoma

  • Amornrat Juasook
  • Ratchadawan Aukkanimart
  • Thidarut Boonmars
  • Pakkayanee Sudsarn
  • Nadchanan Wonkchalee
  • Porntip Laummaunwai
  • Pranee Sriraj
Research

Abstract

The results of a previous study demonstrated that prednisolone enhanced cholangiocarcinogenesis. Therefore, to clarify molecular changes during immunosuppressive cholangiocarcinogenesis, Syrian hamsters were divided into 8 groups: uninfected controls; immunosuppressed Syrian hamsters using prednisolone (P); normal Syrian hamsters administered N-nitrosodimethylamine (ND); immunosuppressed Syrian hamsters administered N-nitrosodimethylamine (NDis); normal Syrian hamsters infected with Opisthorchis viverrini (OV); immunosuppressed Syrian hamsters infected with O. viverrini (OVis); normal Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCA); and immunosuppressed Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCAis). Syrian hamster livers were used for analysis of tumor-related gene expression and immunohistochemistry through cytokeratin 19 (CK19) and proliferating cell nuclear antigen (PCNA) staining. The tumor-related gene expression results show that CCAis groups at all time points exhibited upregulation of COX-2, IL-6, SOD1, CAT and iNOS and downregulation of p53, which correlated with the predominant expression of CK19 and PCNA in liver tissue. These results suggest that prednisolone enhances cholangiocarcinoma development, which was confirmed by molecular changes.

Keywords

Immunosuppressive Cholangiocarcinogenesis Tumor-related gene Immunohistochemistry 

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Copyright information

© Arányi Lajos Foundation 2013

Authors and Affiliations

  • Amornrat Juasook
    • 1
    • 2
    • 3
  • Ratchadawan Aukkanimart
    • 1
    • 2
  • Thidarut Boonmars
    • 1
    • 2
  • Pakkayanee Sudsarn
    • 1
    • 2
  • Nadchanan Wonkchalee
    • 1
    • 2
  • Porntip Laummaunwai
    • 1
  • Pranee Sriraj
    • 1
    • 2
  1. 1.Department of Parasitology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  2. 2.Liver Fluke and Cholangiocarcinoma Research CenterKhon Kaen UniversityKhon KaenThailand
  3. 3.Faculty of Veterinary SciencesMahasarakham UniversityMahasarakhamThailand

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