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XRCC2 R188H (rs3218536), XRCC3 T241M (rs861539) and R243H (rs77381814) Single Nucleotide Polymorphisms in Cervical Cancer Risk

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Pathology & Oncology Research

Abstract

Human Papillomavirus (HPV) is the main cause of cervical cancer and its precursor lesions. Transformation may be induced by several mechanisms, including oncogene activation and genome instability. Individual differences in DNA damage recognition and repair have been hypothesized to influence cervical cancer risk. The aim of this study was to evaluate whether the double strand break gene polymorphisms XRCC2 R188H G>A (rs3218536), XRCC3 T241M C>T (rs861539) and R243H G>A (rs77381814) are associated to cervical cancer in Argentine women. A case control study consisting of 322 samples (205 cases and 117 controls) was carried out. HPV DNA detection was performed by PCR and genotyping of positive samples by EIA (enzyme immunoassay). XRCC2 and 3 polymorphisms were determined by pyrosequencing. The HPV-adjusted odds ratio (OR) of XRCC2 188 GG/AG genotypes was OR = 2.4 (CI = 1.1–4.9, p = 0.02) for cervical cancer. In contrast, there was no increased risk for cervical cancer with XRCC3 241 TT/CC genotypes (OR = 0.48; CI = 0.2–1; p = 0.1) or XRCC3 241 CT/CC (OR = 0.87; CI = 0.52–1.4; p = 0.6). Regarding XRCC3 R243H, the G allele was almost fixed in the population studied. In conclusion, although the sample size was modest, the present data indicate a statistical association between cervical cancer and XRCC2 R188H polymorphism. Future studies are needed to confirm these findings.

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Acknowledgments

The authors would like to thank the National Agency of Scientific and Technological Promotion (PICT 01–20149.) for their funding of this research.

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We declare that we have no conflict of interest.

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Authors and Affiliations

  1. IGEVET-CONICET (Institute of Veterinary Genetics-National Council of Scientific and Technical Investigations), Faculty of Veterinary Genetics, University of La Plata, 60th and 118th Street, PC B1900, La Plata, Buenos Aires, Argentina

    Luis Orlando Pérez, Andrea Crivaro, Gisela Barbisan, Lucia Poleri & Carlos Daniel Golijow

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  1. Luis Orlando Pérez
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  2. Andrea Crivaro
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  3. Gisela Barbisan
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  4. Lucia Poleri
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  5. Carlos Daniel Golijow
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Correspondence to Luis Orlando Pérez.

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Pérez, L.O., Crivaro, A., Barbisan, G. et al. XRCC2 R188H (rs3218536), XRCC3 T241M (rs861539) and R243H (rs77381814) Single Nucleotide Polymorphisms in Cervical Cancer Risk. Pathol. Oncol. Res. 19, 553–558 (2013). https://doi.org/10.1007/s12253-013-9616-2

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