Pathology & Oncology Research

, Volume 19, Issue 3, pp 521–527 | Cite as

A-Kinase Anchoring Proteins 10 Expression in Relation to 2073A/G Polymorphism and Tumor Progression in Patients with Colorectal Cancer

  • Mojin Wang
  • Dan Zhang
  • Rui Wang
  • Yuanyi Rui
  • Jin Zhou
  • Rong Wang
  • Bin Zhou
  • Xiaoran Huang
  • Lie Yang
  • Yuan Li
  • Jiankun Hu
  • Zongguang Zhou
  • Xiaofeng Sun
Research

Abstract

The cAMP/PKA signalling events regulated by A-kinase anchoring proteins 10 (AKAP10) is involved in tumorigenesis. Previous study showed that AKAP10 polymorphism (2073 A/G, I646V) was associated with colorectal cancer risk. However, there was no literature reporting the role of AKAP10 in the pathogenesis of colorectal cancer. The aim of the study was to investigate the clinicopathologic significance of A-kinase anchoring proteins 10 (AKAP 10) expression and the relationship with its polymorphism in colorectal cancer. The expression of AKAP10 was determined by immunohistochemical staining (IHC) and western blot assay on colorectal cancer (n = 176), adenoma (n = 87) and distant normal mucosa (n = 72). 176 patients with colorectal cancer were genotyped for AKAP10 2073A/G polymorphism by TaqMan RT-PCR. We found that the positive expression rate of AKAP10 in colorectal cancer (59 %) was significantly higher than those in adenoma (39 %) and distant normal mucosa (42 %) (P = 0.004). There was no significant difference between adenoma and distant normal mucosa (P = 0.741). Positive AKAP10 staining was correlated with deeper tumor invasion (P < 0.001), lymph nodes metastasis (P = 0.022), advanced tumor stage (P < 0.001) and poorly differentiated degree (P = 0.003). Compared with AA genotype (52 %), positive expression of AKAP10 was significantly increased in colorectal cancer patients with the variant (AG+GG) genotypes (68 %, P = 0.033). It was concluded that AKAP10 may play an important role in the development and progression of colorectal cancer.

Keywords

A-kinase anchoring proteins 10 Immunohistochemical staining Western blot Polymorphism Colorectal cancer 

Notes

Acknowledgments

We thank Professors Zhou and Sun for guiding this study, the members of Digestive Surgery Institution of West China Hospital for useful assistance and colleagues of the Department of Gastrointestinal Surgery for providing experimental specimens. This study was supported by the National Natural Science Founding of China (No. 30830100).

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Copyright information

© Arányi Lajos Foundation 2013

Authors and Affiliations

  • Mojin Wang
    • 1
  • Dan Zhang
    • 1
  • Rui Wang
    • 2
  • Yuanyi Rui
    • 1
  • Jin Zhou
    • 1
  • Rong Wang
    • 1
  • Bin Zhou
    • 1
  • Xiaoran Huang
    • 3
  • Lie Yang
    • 1
  • Yuan Li
    • 4
  • Jiankun Hu
    • 1
  • Zongguang Zhou
    • 1
  • Xiaofeng Sun
    • 5
  1. 1.Department of Gastrointestinal SurgeryInstitute of Digestive Surgery, National Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityChengduChina
  2. 2.Department of GastroenterologyWest China Hospital, Sichuan UniversityChengduChina
  3. 3.Department of PathologyWest China Hospital, Sichuan UniversityChengduChina
  4. 4.Department of Pediatric SurgeryInstitute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityChengduChina
  5. 5.Division of Oncology, Department of Clinical and Experimental MedicineFaculty of Health Sciences, University of LinköpingLinköpingSweden

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