Pathology & Oncology Research

, Volume 17, Issue 4, pp 843–851

Lymphatic Differentiation in Classic Kaposi’s Sarcoma: Patterns of D2-40 Immunoexpression in the Course of Tumor Progression

  • Nilufer Onak Kandemir
  • Figen Barut
  • Banu Dogan Gun
  • Sevinc Hallac Keser
  • Nimet Karadayi
  • Mustafa Gun
  • Sukru Oguz Ozdamar
Research

Abstract

The recent development of lymphatic endothelium-specific immuno-indicators has given rise to research on the histogenesis of Kaposi sarcoma (KS), specifically focusing on its lymphatic root and differentiation. D2-40 is a new lymphatic marker that recognizes podoplanin and is easily applied to formalin-fixed paraffin-embedded human tissues. This study examined D2-40 immunoexpression in 178 classical KS lesions using immunohistochemical methods. D2-40 immunoexpression was also examined in 63 non-KS soft tissue lesions to test the reliability of D2-40 monoclonal antibody in the pathological diagnosis of KS. D2-40 immunoreactivity was detected at all of the KS lesions and in lymphangioma and nonneoplastic lymphatic endothelium. There was no significant relationship between the extent of D2-40 staining and histopathological stage; however, there was a positive correlation between the staining intensity and histopathological stage in KS cases. D2-40 immunoreactivity was detected at all histopathological stages of KS and may be added to the routine immunohistochemical panel used for the differential diagnosis of KS. Widespread D2-40 protein expression is evidence of a lymphatic origin or the differentiation of neoplastic cells in KS, and D2-40 expression increases with tumor progression.

Keywords

D2-40 Immunohistochemistry Kaposi sarcoma Lymphatic Podoplanin 

Abbreviations

ABC-DAB

Avidin biotin complex-diaminobenzidine

AIDS

Acquired Immune Deficiency Syndrome

factor VIII-ra

factor VIII-related antigen

H&E

hematoxylin and eosin

HHV-8

human herpesvirus 8

IHC

immunohistochemistry

KS

Kaposi sarcoma

LNA-1

latent nuclear antigen-1

mAb

monoclonal antibody

SCs

spindle cells

Copyright information

© Arányi Lajos Foundation 2011

Authors and Affiliations

  • Nilufer Onak Kandemir
    • 1
  • Figen Barut
    • 1
  • Banu Dogan Gun
    • 1
  • Sevinc Hallac Keser
    • 2
  • Nimet Karadayi
    • 2
  • Mustafa Gun
    • 3
  • Sukru Oguz Ozdamar
    • 1
  1. 1.Department of Pathology, School of MedicineZonguldak Karaelmas UniversityKozluTurkey
  2. 2.Department of PathologyLütfi Kirdar Kartal Training and Research HospitalİstanbulTurkey
  3. 3.Department of PathologyAtaturk State HospitalZonguldakTurkey

Personalised recommendations