Pathology & Oncology Research

, 15:703 | Cite as

Differential Expression of Notch Family Members in Astrocytomas and Medulloblastomas

  • Peng Xu
  • Shizhu Yu
  • Rongcai Jiang
  • Chunsheng Kang
  • Guangxiu Wang
  • Hao Jiang
  • Peiyu Pu
Article

Abstract

Notch signaling pathway plays an integral role in determining cell fates in development. Growing evidence demonstrates that Notch signaling pathway has versatile effects in tumorigenesis depending on the tumor type, grade and stage. Notch signaling pathway is deregulated in some brain tumors. To examine the differential expression of Notch family members (Notch1, 2, 3, 4) in human astrocytomas and medulloblastomas, and to evaluate their roles in the development of both tumor types. Immunohistochemical staining and Western blot analysis were used to detect Notch1, 2, 3, 4 expression in tissue microarray and freshly resected tissue samples of normal brain, astrocytomas and medulloblastomas. Notch family members were not expressed or barely detectable in normal brain tissues. Notch1, 3, 4 were highly expressed but Notch2 was not expressed in astrocytomas. The percentage of immunopositive tumor cells and level of Notch1 expression was increased with tumor grade. In addition, overexpression of Notch2 was detected in medulloblastomas in contrast to low or no expression of Notch1, 3, 4. Differential expression of Notch1, 2, 3, 4 is detected in astrocytomas and medulloblastomas, that may be related to their different roles playing in the development of brain tumors.

Keywords

Astrocytomas Differential expression Medulloblastomas Notch family members 

Abbreviations

AST

astrocytomas

CBF

C promoter binding factor

CIR

CBF-interacting repressor

CSL

CBF-1/RBPJk-Su (H)-LAG1

EGL

external granule layer

GNPs

granule neuron precursors

HES

hairy-enhancer of split

HERP

HES-related repressor proteins

MAML-1

mastermind-like 1

MB

medulloblastomas

NICD

notch intracellular domain

pCAF

P300/CBP-associated factor

PEST

proline-glutamate-serine-threonine-rich

PNET

primitive neuroectodermal tumors

RAM

RBP-JK-associated molecule

SAGE

serial analysis of gene expression

SMRT

silencing mediator of retinoid and thyroid receptors

TAD

transcription transactivation domain

T-ALLs

T cell acute lymphoblastic leukemias

Notes

Acknowledgement

This work is supported by National Natural Science Foundation of China (Grant number 30300365), Tianjin Science and Technology Committee (Grant Number 06YFSZSF01100)

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Copyright information

© Arányi Lajos Foundation 2009

Authors and Affiliations

  • Peng Xu
    • 1
  • Shizhu Yu
    • 2
  • Rongcai Jiang
    • 1
  • Chunsheng Kang
    • 1
  • Guangxiu Wang
    • 1
  • Hao Jiang
    • 3
  • Peiyu Pu
    • 1
    • 4
  1. 1.Department of Neurosurgery, Laboratory of Neuro-Oncology, Tianjin Neurological InstituteTianjin Medical University General HospitalTianjinPeople’s Republic of China
  2. 2.Laboratory of NeuropathologyTianjin Neurological InstituteTianjinChina
  3. 3.Department of NeurologyHenry Ford HospitalDetroitUSA
  4. 4.Department of NeurosurgeryTianjin Medical University General HospitalTianjinPeople’s Republic of China

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