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Pathology & Oncology Research

, 15:533 | Cite as

Zonula Occludens-1, Occludin, and E-cadherin Protein Expression in Biliary Tract Cancers

  • Zsuzsanna Németh
  • Attila Marcell Szász
  • Áron Somorácz
  • Péter Tátrai
  • Júlia Németh
  • Hajnalka Győrffy
  • Attila Szíjártó
  • Péter Kupcsulik
  • András Kiss
  • Zsuzsa SchaffEmail author
Original Paper

Abstract

The incidence of cholangiocarcinomas originating from intrahepatic and extrahepatic bile ducts, as well as of gallbladder carcinoma is increasing worldwide. The malignant transformation of biliary epithelia involves profound alterations of proteins in the intercellular junctions, among others zonula occludens-1 (ZO-1), occludin, and E-cadherin. Each plays important role in the maintenance of epithelial cell polarity and regulation of cell growth and differentiation. Our aim was to investigate ZO-1, occludin, and E-cadherin immunohistochemical reactions in tissue microarray blocks containing 57 normal and 62 neoplastic biliary tract samples. We demonstrated that the tight junction components ZO-1, occludin, and E-cadherin are downregulated in carcinomas arising from various compartments of the biliary tract (normal intrahepatic and extrahepatic bile ducts, gallbladder) as compared with their normal sites of origin. These results were confirmed by discriminant analysis yielding clear separation of the three normal sample groups from carcinomas in the corresponding locations.

Keywords

Adherens junction Biliary tract cancer Tight junction 

Abbreviations

CCCs

Cholangiocarcinomas

GBCs

gallbladder carcinomas

EBDC

extrahepatic bile duct cancers

IBDC

intrahepatic bile duct cancers

AJ

adherens junctions

TJ

tight junctions

ZO-1

zonula occludens-1

EMT

epithelial-to-mesenchymal transition

FFPE

formalin-fixed, paraffin-embedded

TMA

Tissue Micro-Array

NIBD

normal intrahepatic bile duct

NEBD

normal extrahepatic bile duct

NGB

normal gall bladder

Notes

Acknowledgements

Authors wish to thank Mrs Magdolna Pekár and Mrs Erzsébet Azumah for preparing the TMAs and the immunohistochemical reactions and Mrs Elvira Kale-Rigo for the careful reading and correction of the manuscript.

This work was supported by Grant 75468 from the Hungarian Scientific Research Fund (OTKA).

Conflict of interest statement

The authors of the present study confirm that there is no conflict of interest to be declared.

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Copyright information

© Arányi Lajos Foundation 2009

Authors and Affiliations

  • Zsuzsanna Németh
    • 1
  • Attila Marcell Szász
    • 1
  • Áron Somorácz
    • 1
  • Péter Tátrai
    • 1
  • Júlia Németh
    • 1
  • Hajnalka Győrffy
    • 1
  • Attila Szíjártó
    • 2
  • Péter Kupcsulik
    • 2
  • András Kiss
    • 1
  • Zsuzsa Schaff
    • 1
    Email author
  1. 1.2nd Department of PathologySemmelweis UniversityBudapestHungary
  2. 2.1st Department of SurgerySemmelweis UniversityBudapestHungary

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