Overexpression of CD24, c-myc and Phospholipase 2A in Prostate Cancer Tissue Samples Obtained by Needle Biopsy

Article

Abstract

Altered CD24, c-myc and phospholipase 2a expression was reported in different cancers. Our aim was to measure the expression of these genes in prostate cancer tissues, and compare it to non-cancerous samples. Prostate tissue samples were collected by needle biopsy from 20 prostate cancer (PCA) and 11 benign prostate hyperplasic (BPH) patients. RNA was isolated; cDNA synthetized, CD24, c-myc and phospholipase 2A (PL2A) expressions were determined by quantitative real-time PCR method. The expression of β-globin gene was measured for normalization of the gene expression results. Serum prostate specific antigen (PSA) levels were determined by microparticle enzyme immunoassay (MEIA) method. PSA levels were significantly different between the PCA and BPH groups, 252.37 ± 308.33 ng/ml vs. 3.5 ± 2.14 ng/ml (p = 0.001), respectively. CD24 expression was 988.86 ± 3041 ng/μl in prostate tumor and 4.00 ± 4.25 ng/μl in the BPH group (p = 0.035). The c-myc expression was 88.32 ± 11.93 ng/μl in the prostate tumor and 17.08 ± 21.75 ng/μl in the BPH group (p = 0.02), and the PL2A 31.36 ± 67.02 ng/μl was in PCA and 5.56 ± 14.08 ng/μl in BPH (p = 0.025). Gleason’s scores showed correlation with c-myc (p = 0.019) and PSA (p = 0.033) levels. Overexpression of PL2A, CD24 and c-myc was observed in prostate cancer samples using quantitative real-time PCR method.

Keywords

Prostate cancer Gene expression CD24 c-myc Phospholipase 2A 

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Copyright information

© Arányi Lajos Foundation 2008

Authors and Affiliations

  1. 1.Genetic Laboratory, 1st Department of Obstetrics and GynecologySemmelweis UniversityBudapestHungary
  2. 2.Department of UrologySemmelweis UniversityBudapestHungary
  3. 3.BudapestHungary

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