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Pathology & Oncology Research

, Volume 14, Issue 1, pp 15–22 | Cite as

Expression of Matrilin-2 in Liver Cirrhosis and Hepatocellular Carcinoma

  • Erzsébet Szabó
  • Éva Korpos
  • Enkhjargal Batmunkh
  • Gábor Lotz
  • Ágnes Holczbauer
  • Ilona Kovalszky
  • Ferenc Deák
  • Ibolya Kiss
  • Zsuzsa SchaffEmail author
  • András Kiss
Original Paper

Abstract

The recently described matrilin protein family is part of the extracellular matrix, their pathophysiological role as well as distribution in liver diseases, however, have not yet been studied. Considering that matrilins have been found to play role in cell growth and tissue remodeling, their possible involvement in carcinogenesis has been raised. The main objective of this study was to investigate the changes in matrilin-2 expression which is one of the main components of basement membranes. Thirty-five cases of surgically resected hepatocellular carcinomas, 35 corresponding surrounding liver tissues and 10 normal liver samples were used for the study. In 15 of 35 cases the tumor developed on the basis of cirrhosis. Matrilin-2 protein expression was detected in normal liver around bile ducts, portal blood vessels, while sinusoids were negative by immunohistochemistry. Cirrhotic surrounding tissue showed intensive matrilin-2 staining along the sinusoids. Tumorous neovasculature was found strongly positive by immunohistochemistry. No differences, however, were detected by morphometry regarding the amount of protein expression based on the grade of hepatocellular carcinomas. Real-time RT-PCR did not show significant differences in matrilin-2 mRNA expression between normal, cirrhotic and tumor samples. This suggests posttranslational modification of matrilin-2 manifesting in altered distribution in liver fibrosis. Our data indicate that matrilin-2 is a novel basement membrane component in the liver, which is synthetised during sinusoidal “capillarization” in cirrhosis and in hepatocellular carcinoma. This is the first report to describe the expression and distribution of matrilin-2 in human normal and cirrhotic liver as well as in hepatocellular carcinoma.

Keywords

Matrilin-2 Basement membrane Extracellular matrix Hepatocellular carcinoma Cirrhosis 

Abbreviations

HCC

hepatocellular carcinoma

ECM

extracellular matrix

Notes

Acknowledgments

The authors thank Magdolna Pekár, Ágnes Szík for their skilful technical assistance.

This study was supported by the following grants: NKFP-1A /002/056/2004, ETT-049/2006, ETT-156/2006, ETT 008/2006 from the Ministry of Health and OTKA-T049559, OTKA T049608 from the Hungarian National Scientific Research Foundation.

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Copyright information

© Arányi Lajos Foundation 2008

Authors and Affiliations

  • Erzsébet Szabó
    • 1
  • Éva Korpos
    • 2
  • Enkhjargal Batmunkh
    • 1
  • Gábor Lotz
    • 1
  • Ágnes Holczbauer
    • 1
  • Ilona Kovalszky
    • 3
  • Ferenc Deák
    • 2
  • Ibolya Kiss
    • 2
  • Zsuzsa Schaff
    • 1
    Email author
  • András Kiss
    • 1
  1. 1.2nd Department of PathologySemmelweis UniversityBudapestHungary
  2. 2.Institute of BiochemistryBiological Research Center of the Hungarian Academy of SciencesSzegedHungary
  3. 3.1st Institute of Pathology and Experimental Cancer ResearchSemmelweis UniversityBudapestHungary

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