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Genome Analysis of Dasineura jujubifolia Toursvirus 2, A Novel Ascovirus

  • Jun Wang
  • Minglu YangEmail author
  • Haibing Xiao
  • Guo-Hua Huang
  • Fei Deng
  • Zhihong HuEmail author
RESEARCH ARTICLE

Abstract

So far, ascoviruses have only been identified from Lepidoptera host insects and their transmission vectors—endoparasitic wasps. Here, we reported the first finding of a complete novel ascovirus genome from a Diptera insect, Dasineura jujubifolia. Initially, sequence fragments with homology to ascoviruses were incidentally identified during metagenomic sequencing of the mitochondria of D. jujubifolia (Cecidomyiidae, Diptera) which is a major pest on Ziziphus jujuba. Then a full circular viral genome was assembled from the metagenomic data, which has an A+T percentage of 74% and contains 142,600 bp with 141 open reading frames (ORFs). Among the 141 ORFs, 37 were conserved in all sequenced ascoviruses (core genes) including proteins predicted to participate in DNA replication, gene transcription, protein modification, virus assembly, lipid metabolism and apoptosis. Multi-gene families including those encode for baculovirus repeated open reading frames (BROs), myristylated membrane proteins, RING/U-box E3 ubiquitin ligases, and ATP-binding cassette (ABC) transporters were found in the virus genome. Phylogenetic analysis showed that the newly identified virus belongs to genus Toursvirus of Ascoviridae, and is therefore named as Dasineura jujubifolia toursvirus 2 (DjTV-2a). The virus becomes the second reported species of the genus after Diadromus pulchellus toursvirus 1 (DpTV-1a). The genome arrangement of DjTV-2a is quite different from that of DpTV-1a, suggesting these two viruses separated in an early time of evolution. The results suggest that the ascoviruses may infect a much broader range of hosts than our previous knowledge, and shed lights on the evolution of ascoviruses and particularly on that of the toursviruses.

Keywords

Dasineura jujubifolia toursvirus 2 (DjTV-2a) Ascovirus Toursvirus 

Notes

Acknowledgements

This work was supported by the International Science and Technology Cooperation Program of Xinjiang Construction Corps (Grant No. 2017BC004) and the National Natural Science Foundation of China (Grant No. 31900154).

Author Contributions

HX and MY conceived and designed the experiments. HX performed the experiments. JW, ZH, GHH analysis the data. JW, FD, ZH, GHH, and MY wrote the manuscript. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interests.

Animal and Human Rights Statement

This article does not contain any studies with human or animal subjects performed by any of the authors.

Supplementary material

12250_2019_177_MOESM1_ESM.pdf (295 kb)
Supplementary material 1 (PDF 295 kb)
12250_2019_177_MOESM2_ESM.xlsx (5.6 mb)
Supplementary material 2 (XLSX 5756 kb)

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Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.Tarim UniversityAlarChina
  3. 3.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Institute of VirologyHunan Agricultural UniversityChangshaChina

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