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Virologica Sinica

, Volume 34, Issue 6, pp 610–617 | Cite as

The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication

  • Qiao Xue
  • Huisheng Liu
  • Qiaoying ZengEmail author
  • Haixue Zheng
  • Qinghong Xue
  • Xuepeng Cai
Research Article
  • 306 Downloads

Abstract

Foot-and-mouth disease virus (FMDV) can infect domestic and wild cloven-hoofed animals. The non-structural protein 3D plays an important role in FMDV replication and pathogenesis. However, the interaction partners of 3D, and the effects of those interactions on FMDV replication, remain incompletely elucidated. In the present study, using the yeast two-hybrid system, we identified a porcine cell protein, DEAD-box RNA helicase 1 (DDX1), which interacted with FMDV 3D. The DDX1-3D interaction was further confirmed by co-immunoprecipitation experiments and an indirect immunofluorescence assay (IFA) in porcine kidney 15 (PK-15) cells. DDX1 was reported to either inhibit or facilitate viral replication and regulate host innate immune responses. However, the roles of DDX1 during FMDV infection remain unclear. Our results revealed that DDX1 inhibited FMDV replication in an ATPase/helicase activity-dependent manner. In addition, DDX1 stimulated IFN-β activation in FMDV-infected cells. Together, our results expand the body of knowledge regarding the role of DDX1 in FMDV infection.

Keywords

Foot-and-mouth disease virus (FMDV) Interaction DEAD-box RNA helicase 1 (DDX1) Antiviral function Interferon 

Notes

Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (Nos. 31302106, 31260616, and 31602035); and the National Key Research and Development Program of China (Nos. 2016YFD0500901 and 2017YFD0500903).

Author Contributions

QZ, XC, and HZ designed the experiments. QX and HL carried out the experiments. QX, HL, and QHX analyzed the data. QX and HL wrote the paper. All the authors approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

This article does not contain any studies with human or animal subjects performed by any of the authors.

Supplementary material

12250_2019_148_MOESM1_ESM.pdf (398 kb)
Supplementary material 1 (PDF 398 kb)

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Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.Laboratory of Veterinary Microbiology, College of Veterinary MedicineGansu Agricultural UniversityLanzhouChina
  2. 2.State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouChina
  3. 3.China Institute of Veterinary Drug ControlBeijingChina

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