Virologica Sinica

, Volume 34, Issue 3, pp 338–341 | Cite as

Reverse Transcription Recombinase Polymerase Amplification Assays for Rapid Detection of Tick-Borne Encephalitis Virus Infection

  • Jia Jia
  • Yuchang Li
  • Xiaoyan Wu
  • Sen Zhang
  • Yi Hu
  • Jing Li
  • Tao JiangEmail author
  • Xiaoping KangEmail author

Dear Editor,

Tick-borne encephalitis virus (TBEV) belongs to the genus Flavivirus within the family Flaviviridae and includes three subtypes: Siberian, European, and Far Eastern. TBEV infects humans via tick bites; indeed, at least 10,000 human cases of encephalitis caused by TBEV are reported annually in Russia, China, and European countries (Banzhoff et al. 2008; Carletti et al. 2017).

In China, the main subtype of TBEV is Far Eastern (Zhang et al. 2016).This subtype spread across the forest region of northeast and northwest China, with thousands of people infected annually. TBEV mainly causes severe meningitis and encephalitis. The symptoms include fever, headache, neurological disorders, and/or peripheral flaccid paralysis (Mansfield et al. 2009). Early diagnosis is crucial for the control and treatment of TBEV infection.

Currently, no commercial assay kits for TBEV infection are available in China. Hospitals most commonly use enzyme-linked immune absorbent assays and...



This work was supported by the National Major Science Program Foundation (Nos. 2018ZX10711001-003, 2018ZX10302401-008 and 2017ZX10305501-010), the Major Special Program Foundation (No. AWS15J006), and the National China Science Foundation (No. 81501789).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

This study was approved by the ethics committee of Beijing Institute of Microbiology and Epidemiology. Informed consent was obtained from all of the patients whose sera or CSF samples were used in this study.

Supplementary material

12250_2019_105_MOESM1_ESM.pdf (535 kb)
Supplementary material 1 (PDF 536 kb)


  1. Banzhoff A, Bröker M, Zent O (2008) Protection against tick-borne encephalitis (TBE) for people living in and travelling to TBE-endemic areas. Travel Med Infect Dis 6:331–341CrossRefGoogle Scholar
  2. Carletti T, Zakaria MK, Marcello A (2017) The host cell response to tick-borne encephalitis virus. Biochem Biophys Res Commun 492:533–540CrossRefGoogle Scholar
  3. Eulera M, Wangb Y, Nentwichc O, Piepenburgc O, Huferta FT, Weidmann M (2012) Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus. J Clin Virol 54:308–312CrossRefGoogle Scholar
  4. Maffert P, Reverchon S, Nasser W, Rozand C, Abaibou H (2017) New nucleic acid testing devices to diagnose infectious diseases in resource-limited settings. Eur J Clin Microbiol Infect Dis 36:1717–1731CrossRefGoogle Scholar
  5. Mansfield KL, Johnson N, Phipps LP, Stephenson JR, Fooks AR, Solomon T (2009) Tick-borne encephalitis virus—a review of an emerging zoonosis. J Gen Virol 90:1781–1794CrossRefGoogle Scholar
  6. Piepenburg O, Williams CH, Stemple DL, Armes NA (2006) DNA detection using recombination proteins. PLoS Biol 4:e204CrossRefGoogle Scholar
  7. Schwaiger M, Cassinotti P (2003) Development of a quantitative real-time RT-PCR assay with internal control for the laboratory detection of tick borne encephalitis virus (TBEV) RNA. J Clin Virol 27:136–145CrossRefGoogle Scholar
  8. Zhang X, Zheng Z, Shu B, Mao P, Bai B, Hu Q, Cui Z, Wang H (2016) Isolation and characterization of a Far-Eastern strain of tick-borne encephalitis virus in China. Virus Res 213:6–10CrossRefGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyBeijingChina
  2. 2.Center for Disease Control and Prevention of Shunyi DistrictBeijingChina
  3. 3.Graduate SchoolAnhui Medical UniversityHefeiChina

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