Serological Cross Reactivity between Zika and Dengue Viruses in Experimentally Infected Monkeys
Zika virus (ZIKV), a pathogen within the genus Flavivirus, family Flaviviridae brought an international public health emergency due to its association with neonatal microcephaly case (Platt and Miner 2017). Currently the most reliable diagnostic test is PCR detection of ZIKV RNA from body fluid samples. Unfortunately, the short viremia window and asymptomatic/mild infections greatly reduce the success rate of PCRs (de Vasconcelos et al.2018). Serological assays play a pivotal role in retrospective diagnosis of ZIKV infection, especially for pregnant women concerned about ZIKV infection during their early stages of pregnancy (de Vasconcelos et al.2018). However, due to the high level of cross reactivity among related flaviviruses, all serology tests conducted in locations where flaviviruses co-circulate prove to be difficult for making a conclusive diagnosis, especially for diagnosis of ZIKV infection in dengue virus (DENV) pre-exposed individuals (David et al.2017;...
We thank Edgar Macabe Pena and his team, especially Ryan Cabo Maniquiz and Redmond Belen Cheng, for assistance with monkey infection studies. We thank Veronika von Messling for providing expertise and assistance in planning of the animal studies, and Eng Eong Ooi and his lab members, especially Eugenia Ong and Esther Gan, for providing DENV2 viruses. The project was funded in part by NMRC Grant ZRRF16006 and CD-PHRG Grant CDPHRG/0006/2014.
Compliance with Ethical Standards
Conflicts of interest
The authors declare that there are no conflicts of interest.
Animal and Human Rights Statement
Cynomolgus macaques (Macaca fascicularis) were used for all studies. The experiments were approved by the SingHealth Institutional Animal Care and Use Committee of the SingHealth Experimental Medicine Centre (SEMC).
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