Tandem 3′ UTR Patterns and Gene Expression Profiles of Marc-145 Cells During PRRSV Infection
Porcine reproductive and respiratory syndrome virus (PRRSV) causes substantial economic losses to the global pig industry. Alternative polyadenylation (APA) is a mechanism that diversifies gene expression, which is important for tumorigenesis, development, and cell differentiation. However, it is unclear whether APA plays a role in the course of PRRSV infection. To address this issue, in this study we carried out a whole-genome transcriptome analysis of PRRSV-infected Marc-145 African green monkey kidney cells and identified 185 APA switching genes and 393 differentially expressed genes (DEGs). Most of these genes were involved in cellular process, metabolism, and biological regulation, and there was some overlap between the two gene sets. DEGs were found to be more directly involved in the antiviral response than APA genes. These findings provide insight into the dynamics of host gene regulation during PRRSV infection and a basis for elucidating the pathogenesis of PRRSV.
KeywordsPorcine reproductive and respiratory syndrome virus (PRRSV) Alternative polyadenylation (APA) Differentially expressed genes (DEGs) Whole-genome transcriptome analysis
This work was supported by the Natural Science Foundation of Guangdong Province (2014A030312011) and Guangzhou Science and Technology Plan (201804020039).
YC, YW, JL and CX designed the study; YW performed the experiments; JL and YW analyzed the data; YW prepared the figures and tables; YW and YZ wrote the main manuscript. YC checked and finalized the manuscript. All authors read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
This article does not contain any studies with human or animal subjects performed by any of the authors.
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