Virologica Sinica

, Volume 32, Issue 4, pp 307–316 | Cite as

Evaluation of purified recombinant spike fragments for assessment of the presence of serum neutralizing antibodies against a variant strain of porcine epidemic diarrhea virus

  • Jianwei Hao
  • Yun Zhang
  • Shengkun Fang
  • Zhifen Wen
  • Xiangbin Zhang
  • Chunyi Xue
  • Yongchang CaoEmail author
Research Article


Since 2010, variant strains of porcine epidemic diarrhea virus (PEDV) have caused disasters in the pork industry. The spike (S) protein, as the major immunity-eliciting antigen, has previously been used for serological testing and has been found to correlate significantly with the results of the serum neutralization (SN) test. However, further evaluation of this method is needed as new epidemic strains of PEDV emerge. Hence, the main objective of this study was to assess sow sera and determine the correlation between enzyme-linked immunosorbent assay (ELISA) results (involving a newly isolated GDS01 virus-based ELISA and ELISAs based on seven recombinant fragments comprising overlapping S1 and partial S2 sequences) and SN titers. Furthermore, we determined the reliability of the ELISAs based on receiver operating characteristics (ROC) curve analyses. For the most promising ELISA, i.e., the SP4 ELISA, the correlation coefficient (r) and the area under curve (AUC) were determined to be 0.6113 and 0.8538, respectively. In addition, we analyzed the homology of the SP4 sequences obtained from different strains (including vaccine strains) and found that various strains showed a high degree of homology in this region. Thus, we conclude that SP4 is a promising serological testing protein for use in the field.


Porcine epidemic diarrhea virus (PEDV) spike protein enzyme-linked immunosorbent assay serum neutralization test 



This work was supported by the National Key Research and Development Program (2016YFD0500101).

Author Contributions

JH and YZ designed the experiments. JH, YZ, ZW, and XZ carried out the experiments. SF analyzed the data. JH and YZ wrote the manuscript. YC supervised the study and finalized the manuscript. All authors read and approved the final manuscript.

Compliance with Ethics Guidelines

The authors declare that they have no conflict of interest. The animal study was supervised by the Institutional Animal Care and Use Committee of the Sun Yat-sen University (IACUC DD-17-0403) and used in accordance with regulation and guidelines of this committee.

Supplementary material

12250_2017_3969_MOESM1_ESM.pdf (1.1 mb)
Evaluation of purified recombinant spike fragments for assessment of the presence of serum neutralizing antibodies against a variant strain of porcine epidemic diarrhea virus


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Copyright information

© Wuhan Institute of Virology, CAS and Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.State Key Laboratory of Biocontrol, School of Life SciencesSun Yat-sen UniversityGuangzhouChina
  2. 2.Fraunhofer-Institut für Angewandte Informationstechnik FITSankt AugustinGermany

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