Virologica Sinica

, Volume 32, Issue 1, pp 89–96 | Cite as

Isolation, characterization, and phylogenic analysis of three new severe fever with thrombocytopenia syndrome bunyavirus strains derived from Hubei Province, China

  • Yanfang Zhang
  • Shu Shen
  • Junming Shi
  • Zhengyuan Su
  • Mingyue Li
  • Wenjing Zhang
  • Mengmeng Li
  • Zhihong Hu
  • Cheng Peng
  • Xin Zheng
  • Fei Deng
Research article

Abstract

Hubei Province is a major epidemic area of severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) in China. However, to date, a few SFTSV strains have been isolated from Hubei Province, preventing effective studies of epidemic outbreaks. Here, we report three confirmed patients (2015–2016) with typical symptoms of severe fever with thrombocytopenia syndrome disease (SFTS) who were farmers resident in different regions in Hubei Province. Three new SFTSV strains were isolated from the serum samples of each patient. Characterization of viral growth properties showed that there were no significant differences in virus production. All strains were completely sequenced, and phylogenetic analysis showed that unlike the other strains from Hubei province, which belonged to the SFTSV C3 genotype, one of the three strains belonged to the SFTSV C2 genotype. These results suggested that multiple SFTSV genotypes have been circulating in Hubei Province, providing insights into SFTSV evolution and improving our understanding of SFTSV prevalence in Hubei Province.

Keywords

bunyavirus severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) isolation phylogenetic analysis genotype 

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Notes

Acknowledgments

This work was supported by the Science and Technology Basic Work Program (2013FY113500) and the National Key Research and Development Program (2016YFE 0113500) from the Ministry of Science and Technology of China, as well as the European Union’s Horizon 2020 EVAg project (No 653316).

Supplementary material

12250_2017_3953_MOESM1_ESM.pdf (88 kb)
Isolation, characterization, and phylogenic analysis of three new severe fever with thrombocytopenia syndrome bunyavirus strains derived from Hubei Province, China

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Copyright information

© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore 2017

Authors and Affiliations

  • Yanfang Zhang
    • 1
  • Shu Shen
    • 1
  • Junming Shi
    • 1
  • Zhengyuan Su
    • 1
  • Mingyue Li
    • 2
  • Wenjing Zhang
    • 2
  • Mengmeng Li
    • 2
  • Zhihong Hu
    • 1
  • Cheng Peng
    • 2
  • Xin Zheng
    • 2
  • Fei Deng
    • 1
  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical CollegeHuaZhong University of Science and TechnologyWuhanChina

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