Genotyping of white spot syndrome virus in Chinese cultured shrimp during 1998–1999
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Recent studies showed that white spot syndrome virus (WSSV) isolates from different geographic locations share a high genetic similarity except the variable regions in ORF23/24 and ORF14/15, and variable number of tandem repeats (VNTR) within ORF94. In this study, genotyping was performed according to these three variable regions among WSSV isolates collected during 1998/1999 from Southern China. These WSSV isolates contain a deletion of 1168, 5657, 5898, 9316 and 11093 bp, respectively in the variable region ORF23/24 compared with WSSV-TW, and a deletion of 4749 or 5622 bp in the variable region ORF14/15 relative to TH-96-II. Four types of repeat units (RUs) (6, 8, 9 and 13 RUs) in ORF94 were detected in these isolates, with the shortest 6 RUs as the most prevalent type. Our results provide important information for a better understanding of the spatio-temporal transmission mode and the WSSV genetic evolution lineage.
Key wordsWSSV Genotyping Variable region ORF23/24 ORF14/15 ORF94, China
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- 5.Hoa T T T, Hodgson R A J, Oanh D T H, et al. 2005. Genotypic variations in tandem repeat DNA segments between ribonucleotide reductase subunit genes of white spot syndrome virus (WSSV) isolates from Vietnam. In: Diseases in Asian Aquaculture V, Fish Health Section (Walker P, Lester R, Bondad-Reantaso, ed.), Asian Fisheries Society, Manila (2005): 339–351.Google Scholar
- 7.Lightner DV. 1996. A handbook of pathology and diagnostic procedures for diseases of penaeid shrimp. In Special publication of the World Aquaculture Society LA: Baton RougeGoogle Scholar
- 17.Vlak J M, Bonami J R, Flegel T W, et al. 2005. Nimaviridae. In VIIIth Report of the International Committee on Taxonomy of Viruses. Edited by C M Fauquet, M A Mayo, J Maniloff, U Desselberger & L A Ball: Elsevier pp: 187–192.Google Scholar