Virologica Sinica

, Volume 25, Issue 5, pp 375–380 | Cite as

Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture

  • Yang-fei Xiang
  • Huai-qiang Ju
  • Shen Li
  • Ying-jun Zhang
  • Chong-ren Yang
  • Yi-fei Wang


A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG.

Key words

1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG) Phyllanthus emblica Euphorbiaceae HBV Antiviral agents 


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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Yang-fei Xiang
    • 1
  • Huai-qiang Ju
    • 1
  • Shen Li
    • 1
  • Ying-jun Zhang
    • 2
  • Chong-ren Yang
    • 2
  • Yi-fei Wang
    • 1
  1. 1.Biomedicine Research & Development CenterJinan UniversityGuangzhouChina
  2. 2.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina

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