Virologica Sinica

, Volume 25, Issue 5, pp 375–380 | Cite as

Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture

  • Yang-fei Xiang
  • Huai-qiang Ju
  • Shen Li
  • Ying-jun Zhang
  • Chong-ren Yang
  • Yi-fei Wang
Article

Abstract

A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG.

Key words

1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG) Phyllanthus emblica Euphorbiaceae HBV Antiviral agents 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Duan D, Li Z, Luo H, et al. 2004. Antiviral compounds from traditional Chinese medicines Galla Chinese as inhibitors of HCV NS3 protease. Bioorg Med Chem Lett, 20; 14(24): 6041–6044.CrossRefGoogle Scholar
  2. 2.
    EL-Mekkawy S, Meselhy M R, Kusumoto I T, et al. 1995. Inhibitory effects of Egyptian folk medicines on human immunodeficiency virus (HIV) reverse transcriptase. Chem Pharm Bull (Tokyo), 43(4): 641–648.PubMedGoogle Scholar
  3. 3.
    Fukuchi K, Sakagami H, Okuda T, et al. 1989. Inhibition of herpes simplex virus infection by tannins and related compounds. Antiviral Res, 11(5–6): 285–297.CrossRefPubMedGoogle Scholar
  4. 4.
    Korba B E, Milman G. 1991. A cell culture assay for compounds which inhibit hepatitis B virus replication. Antiviral Res, 15(3): 217–228.CrossRefPubMedGoogle Scholar
  5. 5.
    Lançon A, Delmas D, Osman H, et al. 2004. Human hepatic cell uptake of resveratrol: involvement of both passive diffusion and carrier-mediated process. Biochem Biophys Res Commun, 316(4): 1132–1137.CrossRefPubMedGoogle Scholar
  6. 6.
    Lançon A, Hanet N, Jannin B, et al. Resveratrol in human hepatoma HepG2 cells: metabolism and inducibility of detoxifying enzymes. Drug Metab Dispos, 35(5): 699–703.Google Scholar
  7. 7.
    Lee WM. 1997. Hepatitis B virus infection. N Engl J Med, 337(24): 1733–1745.CrossRefPubMedGoogle Scholar
  8. 8.
    Lee S J, Lee H K, Jung M K, et al. 2006. In vitro antiviral activity of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose against hepatitis B virus. Biol Pharm Bull, 29(10): 2131–2134.CrossRefPubMedGoogle Scholar
  9. 9.
    Manabu T, Masayuki T, Yasuo T. 1985. Studies on antiviral activity of several hydrolyzable tannins. International congress series (Herpes viruses virus chemother), 667: 223–224.Google Scholar
  10. 10.
    Nakashima H, Ichiyama K, Hirayama F, et al. 1996. Sulfated pentagalloyl glucose (Y-ART-3) inhibits HIV replication and cytopathic effects in vitro, and reduces HIV infection in hu-PBL-SCID mice. Antiviral Res, 30(2–3): 95–108.CrossRefPubMedGoogle Scholar
  11. 11.
    Sun J, Hou J L. 2010. Management of chronic hepatitis B: experience from China. J Viral Hepatitis, 17(S1): 10–17.CrossRefGoogle Scholar
  12. 12.
    Tiollais P, Pourcel C, Dejean A. 1985. The hepatitis B virus. Nature, 317(5): 489–495.CrossRefPubMedGoogle Scholar
  13. 13.
    Xia Q, Xiao P G, Wang L W, et al. 1997. Ethnopharmacology of Phyllanthus emblica L. China J Chin Mat Med, 22(9): 515–518. (in Chinese)Google Scholar
  14. 14.
    Xiang Y F, Pei Y, Wang Y F. 2008. Current status of natural products from plants as anti-herpes simplex virus 1 agents. Virol Sin, 23(5): 305–314.CrossRefGoogle Scholar
  15. 15.
    Yeo S J, Yun Y J, Lyu M A, et al. 2002. Respiratory syncytial virus infection induces matrix metal-loproteinase-9 expression in epithelial cells. Arch Virol, 147(2):229–242.CrossRefPubMedGoogle Scholar
  16. 16.
    Zhang J, Li L, Kim S H, et al. 2009. Anti-cancer, anti-diabetic and other pharmacologic and biological activities of penta-galloyl-glucose. Pharm Res, 26(9): 2066–2080.CrossRefPubMedGoogle Scholar
  17. 17.
    Zhang L, Wang G, Hou W, et al. 2010. Contemporary clinical research of traditional Chinese medicines for chronic hepatitis B in China: an analytical review. Hepatology, 51(2): 690–698.PubMedGoogle Scholar
  18. 18.
    Zhang Y J, Abe T, Tanaka T, et al. 2001. Phyllanemblinins A-F, new ellagitannins from Phyllanthus emblica. J Nat Prod, 64(12): 1527–1532.CrossRefPubMedGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Yang-fei Xiang
    • 1
  • Huai-qiang Ju
    • 1
  • Shen Li
    • 1
  • Ying-jun Zhang
    • 2
  • Chong-ren Yang
    • 2
  • Yi-fei Wang
    • 1
  1. 1.Biomedicine Research & Development CenterJinan UniversityGuangzhouChina
  2. 2.State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina

Personalised recommendations