Virologica Sinica

, Volume 25, Issue 6, pp 440–444

PrP 106-126 Altered PrP mRNA gene expression in mouse microglia BV-2 Cells

  • Yu Bai
  • Yu-rong Li
  • Gui-hua Wang
  • Xiang-mei Zhou
  • De-ming Zhao
Article

Abstract

Prion diseases are infectious and fatal neurodegenerative diseases. The pathogenic agent is an abnormal prion protein aggregate. Microglial activation in the centre nervous system is a characteristic feature of prion disease. In this study, we examined the effect of PrP 106–126 on PrP mRNA gene expression in Mouse microglia cells BV-2 by real-time quantitative PCR. PrP mRNA expression level was found to be significantly increased after 18 h exposure of BV-2 cells to PrP 106–126, with 3-fold increase after 18 h and 4.5-fold increase after 24 h and BV-2 cells proliferating occurred correspondingly. Our results provide the first in vitro evidence of the increase of PrP mRNA levels in microglial cells exposed to PrP 106–126, and indicate that microglial cells might play a critical role in prion pathogenesis.

Key words

Prion PrP106-126 PrP mRNA Mouse microglia BV-2 Cells 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Yu Bai
    • 1
  • Yu-rong Li
    • 2
  • Gui-hua Wang
    • 3
  • Xiang-mei Zhou
    • 1
  • De-ming Zhao
    • 1
  1. 1.The state key Lab of Agrobiotechnology National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary MedicineChina Agricultural UniversityBeijingChina
  2. 2.Department of Animal Science and TechnologyAgricultural University of HebeiBaodingChina
  3. 3.Department of Animal Science and TechnologyAgricultural University of ShandongTaianChina

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