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Virologica Sinica

, Volume 25, Issue 3, pp 177–182 | Cite as

In vivo kinetics and biodistribution of a Hantaan virus DNA vaccine after intramuscular injection in mice

  • Si Wang
  • Qing Nie
  • Lan-yan Zheng
  • Jun Hu
  • En-jie LuoEmail author
Article

Abstract

To study the kinetics in vivo of a Hantaan virus DNA vaccine, we constructed a fusion DNA vaccine, pEGFP/S, by cloning the S segment of Hantavirus into the vector, pEGFP-C1, which encodes Green fluorescent protein EGFP. In this report, we provide evidence that pEGFP/S was distributed and persistently expressed for more than 60 days in several organs after inoculation. Our findings suggest that the persistent immune responses induced by a Hantaan virus DNA vaccine are likely due to the plasmid pEGFP/S deposited in vivo, which acts as a booster immunization.

Key words

Hantaan virus DNA vaccine Intramuscular injection Immunologic memory Nucleocapsid protein 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Si Wang
    • 1
  • Qing Nie
    • 1
  • Lan-yan Zheng
    • 1
  • Jun Hu
    • 1
  • En-jie Luo
    • 1
    Email author
  1. 1.Department of Medical microbiology and parasitology, Preclinical Medical CollegeChina Medical UniversityShenyangChina

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