Virologica Sinica

, 24:470 | Cite as

Field trials of CpGV virus isolates overcoming resistance to CpGV-M

  • M. Berling
  • J. -B. Rey
  • S. -J. Ondet
  • Y. Tallot
  • O. Soubabère
  • A. Bonhomme
  • B. Sauphanor
  • M. Lopez-Ferber
Article

Abstract

The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France and Italy. A laboratory insect colony was started from insects collected in a French resistant orchard. It was named RGV. Various virus isolates were identified as active against this resistant insect colony. Field tests were carried out in 2007 to test if the two virus isolates CpGV-I12 and NPP-R1 were effective in the field. Although these virus isolates were not able to reduce insect caused fruit damages, they significantly reduced the overwintering insect populations. NPP-R1 was subjected to eight passages on RGV larvae (NPP-R1.8) that improved its biological activity on RGV larvae. 2008 field trials were set up to test this improved virus strain, compared to CpGV-I12 and Madex plus active on RGV. These tests confirmed the ability to control both in susceptible and resistant insect populations.

Key words

Cydia pomonella Codling moth Biological control Genotype selection Field trials 

CLC number

R373 

References

  1. 1.
    Asser-Kaiser S, Fritsch E, Undorf-Spahn K, et al. 2007. Rapid emergence of baculovirus resistance in codling moth due to dominant, sex-linked inheritance. Science, 317: 1916–1918.CrossRefPubMedGoogle Scholar
  2. 2.
    Berling M, Blachère-Lopez C, Soubabere O, et al. 2009. Cydia pomonella granulovirus (CpGV) genotypes overcome virus resistance in the codling moth and improve virus efficiency by selection against resistant hosts. Appl Env Microbiol, 75: 925–930.CrossRefGoogle Scholar
  3. 3.
    Eberle K E, Asser-Kaiser S, Sayed S M, et al. 2008. Overcoming the resistance of codling moth against conventional Cydia pomonella granulovirus (CpGV-M) by a new isolate CpGV-I12. J Invertebr Pathol, 98: 293–298.CrossRefPubMedGoogle Scholar
  4. 4.
    Eberle K E, Jehle JA. 2006. Field resistance of codling moth against Cydia pomonella granulovirus (CpGV) is autosomal and incompletely dominant inherited. J Invertebr Pathol, 93: 201–206.CrossRefPubMedGoogle Scholar
  5. 5.
    Fritsch E, Undorf-Spahn K, Kienzle J, et al. 2005. Apfelwickler-Granulovirus: Erste Hinweise auf Unterschiede in der Empfindlichkeit lokaler Apfelwickler-Populationen (Codling moth granulovirus: First indication of variations in the susceptibility of local codling moth populations). Nachrichtenblatt des Deutschen Pflanzenschutzdienstes, 57: 29–34.Google Scholar
  6. 6.
    Huber J. 1986. Use of baculoviruses in pest management programs. In: The biology of Baculoviruses (Granados R R, Federici B A. ed.), Vol II, Fl, Boca Raton: CRC Press. p181–202.Google Scholar
  7. 7.
    Kienzle J, Zimmer J, Volk F, et al. 2008. Field tests with Madex Plus against CpGV-resistant codling moth populations in organic orchards in 2006. In: Ecofruit-13th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing: Proceedings to the Conference (Boos, Markus, Eds), Weinsberg, Germany. p339–349.Google Scholar
  8. 8.
    Lacey L A, Thompson D, Vincent C, et al. 2008. Codding moth granulovirus: a comprehensive review. Biocontrol Sci Techn, 18: 639–663.CrossRefGoogle Scholar
  9. 9.
    Rezapanah M, Shojai-Estabragh S, Hüber J, et al. 2008. Molecular and biological characterization of new isolates of Cydia pomonella granulovirus from Iran. J Pest Sci, 81: 187–191.CrossRefGoogle Scholar
  10. 10.
    Sauphanor B, Berling M, Toubon J F, et al. 2006. Carpocapse des pommes: cas de résistance au virus de la granulose en vergers biologiques. Phytoma, la défense des végétaux, 590: 24–27.Google Scholar
  11. 11.
    Tanada Y. 1964. A granuloses virus of the Codling moth, Carpocapsa pomonella (Linnaeus) (Olethreutidae, Lepidoptera). J Invertebr Pathol, 6: 378–380.Google Scholar
  12. 12.
    Zingg D. 2008. Madex Plus and Madex I12 overcome Virus Resistance of Codling Moth. In: Ecofruit-13th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing: Proceedings to the Conference (Boos, Markus, Eds), Weinsberg, Germany. p256–260.Google Scholar

Copyright information

© Wuhan Institute of Virology, CAS and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • M. Berling
    • 1
  • J. -B. Rey
    • 2
  • S. -J. Ondet
    • 2
  • Y. Tallot
    • 3
  • O. Soubabère
    • 4
  • A. Bonhomme
    • 4
  • B. Sauphanor
    • 5
  • M. Lopez-Ferber
    • 1
  1. 1.Ecole des Mines d’AlèsLGEIAlèsFrance
  2. 2.GRAB. AgroparcAvignon Cedex 9France
  3. 3.Arysta LifeScienceNogueresFrance
  4. 4.NPP, Arysta LifeSciencePauFrance
  5. 5.Unité PSHINRA. AgroparcAvignon Cedex 9France

Personalised recommendations