Virologica Sinica

, Volume 23, Issue 4, pp 272–278 | Cite as

Biological analysis of HSV-1 immediate-early proteins ICP0, ICP22, and ICP27 in neuroblastoma cells

  • Lei Wang
  • Yan-chun Che
  • Wei Cun
  • Wei-zhong Li
  • Yun Liao
  • Long-ding Liu
  • Qi-han LiEmail author


The three immediate-early proteins of HSV-1, ICP0, ICP22, and ICP27, have specific and pivotal functions in transcriptional activation and inhibition, multiple regulatory and control processes of viral genes. In this paper, the expression and localization of these three proteins were studied in neuroblastoma cells using biochemical assays, and their possible and potential interactive functions are discussed. The data show that the three proteins are localized in different structures, specifically in the PML-NB-associated structure, which is a specific nuclear structure composed of many protein molecules and bound tightly to the nuclear matrix in neuroblastoma cells. The results suggest that the activating and suppressive functions of ICPs are mostly dependent on their transcriptional and regulatory roles, including the PML-NB-associated structure.

Key words

Herpes simplex virus type 1 (HSV-1) Immediate-early proteins Human neuroblastoma cells pEGFP Liposome transfection 

CLC number



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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Lei Wang
    • 1
  • Yan-chun Che
    • 1
  • Wei Cun
    • 1
  • Wei-zhong Li
    • 1
  • Yun Liao
    • 1
  • Long-ding Liu
    • 1
  • Qi-han Li
    • 1
    Email author
  1. 1.Peking Union Medical CollegeTsinghua UniversityKunmingChina

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