Virologica Sinica

, Volume 23, Issue 1, pp 73–77 | Cite as

Expression and assembly mechanism of the capsid proteins of a satellite virus (XSV) associated with Macrobrachium rosenbergii nodavirus

Article

Abstract

The extra small virus (XSV) is a satellite virus associated with Macrobrachium rosenbergii nodavirus (MrNV) and its genome consists of two overlapping ORFs, CP17 and CP16. Here we demonstrate that CP16 is expressed from the second AUG of the CP17 gene and is not a proteinase cleavage result of CP17. We further expressed CP17 and several truncated CP17s (in which the N-or C-terminus or both was deleted), respectively, in Escherichia coli. Except for the recombinant plasmid CP17δC10, all recombinant plasmids expressed soluble protein which assembled into virus-like particles (VLPs), suggesting that the C-terminus is important for VLP formation.

Key words

Extra small virus (XSV) Satellite virus Virus-like particle White tail disease 

CLC number

Q938.8 

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References

  1. 1.
    2006. ICTVdB Management. 81. Satellites. In: ICTVdB-The Universal Virus Database (Büchen-Osmond C. ed.), version 3. Columbia University, New York, USA.Google Scholar
  2. 2.
    Anderson I G, Law A T, Shariff M, et al. 1990. A parvo-like in the giant freshwater prawn, Macrobrachium rosenbergii. J Inverte Pathol, 55: 447–449.CrossRefGoogle Scholar
  3. 3.
    Arcier J M, Herman F, Lightner D V, et al. 1999. A viral disease associated with mortalities in hatchery-reared postlarvae of the giant freshwater prawn Macrobrachium rosenbergii. Dis Aquat Org, 38: 177–181.CrossRefGoogle Scholar
  4. 4.
    Bonami J R, Shi Z, Qian D, et al. 2005. White tail disease of the giant freshwater prawn, Macrobrachium rosenbergii: separation of the associated virions and characterization of MrNV as a new type of nodavirus. J Fish Dis, 28: 23–31.PubMedCrossRefGoogle Scholar
  5. 5.
    Gale M, Jr Tan S L, Katze M G. 2000. Translational control of viral gene expression in eukaryotes. Microbiol Mol Biol Rev, 64: 239–280.PubMedCrossRefGoogle Scholar
  6. 6.
    Joshi S M, Vogt V M. 2000. Role of the Rous sarcoma virus p10 domain in shape determination of gag virus-like particles assembled in vitro and within Escherichia coli. J Virol, 74: 10260–10268.PubMedCrossRefGoogle Scholar
  7. 7.
    Majeau N, Gagne V, Boivin A, et al. 2004. The N-terminal half of the core protein of hepatitis C virus is sufficient for nucleocapsid formation. J Gen Virol, 85: 971–981.PubMedCrossRefGoogle Scholar
  8. 8.
    Nash G, Chinabut S, Limsuwan C. 1987. Idopathic muscle necrosis in the freshwater prwan, Macrobrachium rosenbergii de Man, cultured in Thailand. J Fish Dis, 10: 109–120.CrossRefGoogle Scholar
  9. 9.
    Qian D, Shi Z, Zhang S, et al. 2003. Extra small virus-like particles (XSV) and nodavirus associated with whitish muscle disease in the giant freshwater prawn, Macrobrachium rosenbergii. J Fish Dis, 26: 521–527.PubMedCrossRefGoogle Scholar
  10. 10.
    Sahul Hameed A S, Yoganandhan K, Sri Widada J, et al. 2004. Experimental transmission and tissue tropism of Macrobrachium rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV). Dis Aquat Organ, 62: 191–196.PubMedCrossRefGoogle Scholar
  11. 11.
    Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular cloning. A laboratory manual., 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Habor, NY.Google Scholar
  12. 12.
    Shi Z, Qian D, Zhang J, et al. 2004. Isolation, purification and nucleic acid characterization of two viral particles from freshwater prwan Macrobrachium rosenbergii. Chin J Virol, 20: 58–61.Google Scholar
  13. 13.
    Sri Widada J, Bonami J R. 2004. Characteristics of the monocistronic genome of extra small virus, a virus-like particle associated with Macrobrachium rosenbergii nodavirus: possible candidate for a new species of satellite virus. J Gen Virol, 85: 643–646.CrossRefGoogle Scholar
  14. 14.
    Tung C W, Wang C S, Chen S N. 1999. Histological and electron microscopic study on Macrobrachium muscle virus (MMV) infection in the giant freshwater prawn, Macrobrachium rosenbergii (de Man), cultured in Taiwan. J Fish Dis, 22: 319–324.CrossRefGoogle Scholar
  15. 15.
    Zhang H, Wang J, Yuan J, et al. 2006. Quantitative relationship of two viruses (MrNV and XSV) in white-tail disease of Macrobrachium rosenbergii. Dis Aquat Organ, 71: 11–17.PubMedCrossRefGoogle Scholar
  16. 16.
    Zhang H, Yuan J, Qian D, et al. 2004. The Sequencing of an Extra Small Virus Isolated from White-tail-diseased Giant Fresh Water Prawn, Macrobrachium rosenbergii. J Wuhan Univ (Nat. Sci. Ed.), 50: 736–740.Google Scholar

Copyright information

© Wuhan Institute of Virology, CAS 2008

Authors and Affiliations

  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences (CAS)WuhanChina
  2. 2.Graduate School of CASBeijingChina

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