Virologica Sinica

, Volume 22, Issue 4, pp 307–315 | Cite as

Detection and genetic characterization of rabies virus from human patients

  • Wen-rong Yao
  • Guo-qiang Pan
  • Cheng-long Xiong
  • Qian-fu Zhou
  • Qi-you Xiao
  • Ming-hui Li
  • Yong-zhen Zhang
Article

Abstract

Saliva and blood were collected from two patients who had not received post exposure prophylaxis in the cities of Wenzhou and Xinning respectively. Both patients were confirmed as positive for rabies by detection of rabies virus specific nucleoprotein antibodies in the sera by Western Blot. However, rabies virus specific RNA was only identified in the saliva collected from the patient in Wenzhou. Furthermore, the isolate Zhejiang Wz0 (H) was obtained by inoculating one-day-old suckling mice. Both nucleoprotein (N) and glycoprotein (G) genes from the isolate were amplified by RT-PCR and sequenced. Phylogenetic analysis indicated that the isolate belonged to classic rabies virus, and shared a higher homology with the street viruses from dogs in the main endemic areas in China and the street virus from dogs in Indonesia than with other known strains. Further comparison of the deduced amino acid sequences between the isolate and the vaccine strains used in China showed that the virus had a higher level of homology with the vaccine strain CTN than with the other vaccine strains (3aG, PV, PM and ERA). In particular, amino acid residues substitutions located in antigenic site III in the G protein, which could react with the neutralizing antibodies, were observed. These results suggested that the virus belonged to the classic rabies virus, and both N and G genes diverged from the current vaccine strains used in China at either the nucleotide or the amino acid level.

Key words

Rabies Virus Nucleoprotein Gene Glycoprotein Gene Phylogenetic Analysis 

CLC number

R373.9 

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Copyright information

© Wuhan Institute of Virology, CAS 2007

Authors and Affiliations

  • Wen-rong Yao
    • 1
  • Guo-qiang Pan
    • 2
  • Cheng-long Xiong
    • 1
  • Qian-fu Zhou
    • 3
  • Qi-you Xiao
    • 4
  • Ming-hui Li
    • 1
  • Yong-zhen Zhang
    • 1
  1. 1.Department of Hemorrhagic Fever, Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina
  2. 2.The Affiliated Hospital of Wenzhou Medical CollegeWenzhouChina
  3. 3.Xinning Center for Disease Control and PreventionXinningChina
  4. 4.Hunan Center for Disease Control and PreventionChangshaChina

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