Estuaries and Coasts

, Volume 34, Issue 5, pp 925–936

Dissolved Inorganic Nitrogen, Soluble Reactive Phosphorous, and Microbial Pollutant Loading from Tropical Rural Watersheds in Hawai'i to the Coastal Ocean During Non-Storm Conditions

  • Alexandria B. Boehm
  • Kevan M. Yamahara
  • Sarah P. Walters
  • Blythe A. Layton
  • Daniel P. Keymer
  • Rachelle S. Thompson
  • Karen L. Knee
  • Matt Rosener
Article
  • 255 Downloads

Abstract

This study quantifies dissolved inorganic nitrogen (DIN), soluble reactive phosphorous (SRP), and microbial pollutant inputs to a tropical embayment, Hanalei Bay, Kaua'i, Hawai'i from rural watersheds during two field excursions during non-storm conditions. We employ land cover analysis and a suite of nucleic acid fecal source tracking markers (host-specific Bacteroidales and human enterovirus) to identify sources of pollutants to the bay. The highest concentrations of DIN and SRP are in streams draining watersheds with large areas of cultivated land, suggesting fertilizer is a source of these nutrients to the streams and coastal waters. Pollutant areal loading correlates with the fractions of urban and cultivated land cover. Microbial source tracking indicates the presence of human, pig, and ruminant feces in the streams. This work provides preliminary evidence that human development affects loading of DIN, SRP, and microbial pollutants to tropical coastal waters; further study is needed to confirm this. Additionally, results point to a mix of microbial pollutant sources.

Keywords

Microbial pollution Microbial source tracking Tropical streams Inorganic nitrogen Phosphorous Pollutant loading Pollutant flux Bacteroidales Enterovirus Hanalei Bay Hawai'i Rural tropical watersheds Hawaii Enterococci 

References

  1. Ashbolt, N.J., W.O.K. Grabow, and M. Snozzi. 2001. Indicators of microbial water quality. In Water quality: Guidelines, standards and health. risk assessment and management for water-related infectious disease, ed. L. Fewtrell and J. Bartram, 289–315. London: IWA publishing.Google Scholar
  2. Bernhard, A.E., and K.G. Field. 2000a. A PCR assay to discriminate human and ruminant feces on the basis of host differences in Bacteroides–Prevotella genes encoding 16S rRNA. Applied and Environmental Microbiology 66: 4571–4574.CrossRefGoogle Scholar
  3. Bernhard, A.E., and K.G. Field. 2000b. Identification of non-point sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes. Applied and Environmental Microbiology 66: 1587–1594.CrossRefGoogle Scholar
  4. Betancourt, W.Q., and R.S. Fujioka. 2006. Molecular detection of fecal bacteroides 16S rRNA markers in Hawai'i’s stream and coastal waters EOS, Transactions of the American Gynecological. Union 87.Google Scholar
  5. Boehm, A.B., J.A. Fuhrman, R.D. Mrse, and S.B. Grant. 2003. Tiered approach for identification of a human fecal pollution source at a recreational beach: case study at Avalon Bay, Catalina Island, California. Environmental Science & Technology 37: 673–680.CrossRefGoogle Scholar
  6. Boehm, A.B., K.M. Yamahara, D.C. Love, B.M. Peterson, K. Mcneill, and K.L. Nelson. 2009. Covariation and photoinactivation of traditional and novel indicator organisms and human viruses at a sewage-impacted marine beach. Environmental Science & Technology 43: 8046–8052.CrossRefGoogle Scholar
  7. Choi, W.-J., G.-H. Han, S.M. Lee, G.-T. Lee, K.-W. Yoon, S.-M. Choi, and H.-M. Ro. 2007. Impact of land-use types on nitrate concentration and d15N in unconfined groundwater in rural areas of Korea. Agriculture, Ecosystems & Environment 120: 259–268.CrossRefGoogle Scholar
  8. Cole, M.L., I. Valiela, K.D. Kroeger, G.L. Tomasky, J. Cebrian, C. Wigand, R.A. Mckinney, S.P. Grady, and M.H.C.D. Silva. 2004. Assessment of a 15N Method to indicate anthropogenic eutrophication in aquatic ecosystems. Journal of Environmental Quality 33: 124–132.CrossRefGoogle Scholar
  9. Cole, M.L., K.D. Kroeger, J.W. Mcclelland, and I. Valiela. 2006. Effects of watershed land use on nitrogen concentrations and delta-15 nitrogen in groundwater. Biogeochemistry 77: 199–215.CrossRefGoogle Scholar
  10. Derse, E., K.L. Knee, S.D. Wankel, C. Kendall, C.J. Berg, and A. Paytan. 2007. Identifying sources of nitrogen to Hanalei Bay, Kauai, utilizing the nitrogen isotope signature of macroalgae. Environmental Science & Technology 41: 5217–5223.CrossRefGoogle Scholar
  11. Dick, L.K., A.E. Bernhard, T.J. Brodeur, J.W.S. Domingo, J.M. Simpson, S.P. Walters, and K.G. Field. 2005. Host distributions of uncultivated fecal Bacteroides bacteria reveal genetic markers for fecal source identification. Applied and Environmental Microbiology 71: 3184–3191.CrossRefGoogle Scholar
  12. Dorfman, M., and K.S. Rosselot. 2009. Testing the waters: a guide to water quality at vacation beaches. San Francisco: Natural Resources Defense Council.Google Scholar
  13. Endreny, T.A., J.M. Hassett, and S.E. Wolosoff. 2005. Robustness of pollutant loading estimators for sample size reduction in a suburban watershed. International Journal of River Basin Management 3: 53–66.CrossRefGoogle Scholar
  14. Field, K.G., and M. Samadpour. 2007. Fecal source tracking, the indicator paradigm, and managing water quality. Water Research 41: 3517–3538.CrossRefGoogle Scholar
  15. Fuhrman, J.A., X. Liang, and R.T. Noble. 2005. Rapid detection of enteroviruses in small volumes of natural waters by real-time quantitative reverse transcriptase PCR. Applied and Environmental Microbiology 71: 4523–4530.CrossRefGoogle Scholar
  16. Gawler, A.H., J.E. Beecher, J. Brandao, N.M. Carroll, L. Falcao, M. Gourmelon, B. Masterson, B. Nunes, J. Porter, A. Rince, R. Rodrigues, M. Thorp, J.M. Walters, and W.G. Meijer. 2007. Validation of host-specific Bacteriodales 16S rRNA genes as markers to determine the origin of faecal pollution in Atlantic Rim countries of the European Union. Water Research 41: 3780–3784.CrossRefGoogle Scholar
  17. Given, S., L.H. Pendleton, and A.B. Boehm. 2006. Regional public health cost estimates of contaminated coastal waters: a case study of gastroenteritis at Southern California beaches. Environmental Science & Technology 40: 4851–4858.CrossRefGoogle Scholar
  18. Griffith, G.F., S.B. Weisberg, and C.D. Mcgee. 2003. Evaluation of microbial source tracking methods using mixed fecal sources in aqueous test samples. Journal of Water and Health 1: 141–151.Google Scholar
  19. Hardina, C.M., and R.S. Fujioka. 1991. Soil: the environmental source of Escherichia coli and enterococci in Hawai'i’s streams. Environmental Toxicology and Water Quality: An International Journal 6: 185–195.CrossRefGoogle Scholar
  20. Harwood, V.J., J. Whitlock, and V. Withington. 2000. Classification of antibiotic resistance patterns of indicator bacteria by discriminant analysis: use in predicting the source of fecal contamination in subtropical waters. Applied and Environmental Microbiology 66: 3698–3704.CrossRefGoogle Scholar
  21. Kay, D., J. Crowther, C.M. Stapleton, M.D. Wyer, L. Fewtrell, S. Anthony, M. Bradford, A. Edwards, C.A. Francis, M. Hopkins, C. Kay, A.T. Mcdonald, J. Watkins, and J. Wilkinson. 2008. Faecal indicator organism concentrations and catchment export coefficients in the UK. Water Research 42: 2649–2661.CrossRefGoogle Scholar
  22. Kendall, C. 1998. Tracing nitrogen sources and cycling in catchments. In Isotope tracers in catchment hydrology, ed. C. Kendall and J.J. McDonnell, 519–576. Amsterdam: Elsevier.Google Scholar
  23. Kildare, B.J., C.M. Leutenegger, B.S. Mcswain, D.G. Bambic, V.B. Rajal, and S. Wuertz. 2007. 16S rRNA-based assays for quantitative detection of universal, human-, cow-and dog-specific fecal Bacteroidales: a Bayesian approach. Water Research 41: 3701–3715.CrossRefGoogle Scholar
  24. Knee, K.L., B.A. Layton, J. Street, A.B. Boehm, and A. Paytan. 2008. Sources of nutrients and fecal indicator bacteria to nearshore waters on the North Shore of Kaua'i. Estuaries and Coasts 31: 607–622.CrossRefGoogle Scholar
  25. Knee, K.L., J.H. Street, E.G. Grossman, A.B. Boehm, and A. Paytan. 2010. Nutrient inputs to the coastal ocean from submarine groundwater discharge in a groundwater-dominated system: relation to land use (Kona coast, Hawai'i, USA). Limnology and Oceanography 55: 1105–1122.CrossRefGoogle Scholar
  26. Layton, B.A., S.P. Walters, L. Lam, and A.B. Boehm. 2010. Enterococcus species distribution among human and animal hosts using multiplex PCR. Journal of Applied Microbiology. 109: 539–547.Google Scholar
  27. Lewis, W.M., J.M. Melack, W.H. Mcdowell, M. Mcclain, and J.E. Richey. 1999. Nitrogen yields from undisturbed watersheds in the Americas. Biogeochemistry 46: 149–162.CrossRefGoogle Scholar
  28. Liang, K.-Y., and S.L. Zeger. 1986. Longitudinal data analysis using generalized linear models. Biometrika 73: 13–22.CrossRefGoogle Scholar
  29. McCook, L.J. 1999. Macroalgae, nutrients and phase shifts on coral reefs: scientific issues and management consequences for the Great Barrier Reef. Coral Reefs 18: 357–367.CrossRefGoogle Scholar
  30. Mocé-Llivina, L., F. Lucena, and J. Jofre. 2005. Enteroviruses and bacteriophages in bathing waters. Applied and Environmental Microbiology 71: 6838–6844.CrossRefGoogle Scholar
  31. National Research Council. 2000. Clean coastal waters: understanding and reducing the effects of nutrient pollution. Washington, DC: National Academy Press.Google Scholar
  32. Noble, R.T., and J.D. Fuhrman. 2001. Enteroviruses detected by reverse transcriptase polymerase chain reaction from the coastal waters of Santa Monica Bay, California: low correlation to bacterial indicator levels. Hydrobiologia 460: 175–183.CrossRefGoogle Scholar
  33. Parveen, S., K.M. Portier, K. Robinson, L. Edmiston, and M.L. Tamplin. 1999. Discriminant analysis of ribotype profiles of Escherichia coli for differentiating human and nonhuman sources of fecal pollution. Applied and Environmental Microbiology 65: 3142–3147.Google Scholar
  34. Pew Oceans Commission. 2003. America’s living oceans: Charting a course for sea change. A report to the nation. Arlington: Pew Oceans Commission.Google Scholar
  35. Rosario, K., E.M. Symonds, C. Sinigalliano, J. Stewart, and M. Breitbart. 2009. Pepper mild mottle virus as an indicator of fecal pollution. Applied and Environmental Microbiology 75: 7261–7267.CrossRefGoogle Scholar
  36. Santoro, A.E., and A.B. Boehm. 2007. Frequent occurrence of the human-specific Bacteroides fecal marker at an open coast marine beach: Relationship to waves, tides, and traditional indicators. Environmental Microbiology 9: 2038–2049.CrossRefGoogle Scholar
  37. Shanks, O.C., C. Nietch, M. Simonich, M. Younger, D. Reynolds, and K.G. Field. 2006. Basin-wide analysis of the dynamics of fecal contamination and fecal source identification in Tillamook Bay, Oregon. Applied and Environmental Microbiology 72: 5537–5546.CrossRefGoogle Scholar
  38. Shanks, O.C., K. White, C.A. Kelty, S. Hayes, M. Sivaganesan, M. Jenkins, M. Varma, and R.A. Haugland. 2010. Performance assessment PCR-based assays targeting Bacteroidales genetic markers of bovine fecal pollution. Applied and Environmental Microbiology 76: 1359–1366.CrossRefGoogle Scholar
  39. Smart, T.S., D.J. Hirst, and D.A. Elston. 1999. Methods for estimation loads transported by rivers. Hydrology and Earth System Sciences 3: 295–303.CrossRefGoogle Scholar
  40. Sprague, L.A. 2001. Effects of storm-sampling frequency on estimation of water-quality loads and trends in two tributaries to Chesapeake Bay in Virginia. USGS Water-Resources Investigations Report.Google Scholar
  41. Stimson, J., S. Larned, and E. Conklin. 2001. Effects of herbivory, nutrient levels, and introduced algae on the distribution and abundance of the invasive macroalga Dictyosphaeria cavernosa in Kaneohe Bay, Hawai'i. Coral Reefs 19: 343–357.Google Scholar
  42. Stoeckel, D.M., and V.J. Harwood. 2007. Performance, design, and analysis in microbial source tracking studies. Applied and Environmental Microbiology 73: 2405–2415.CrossRefGoogle Scholar
  43. Umezawa, Y., T. Miyajima, M. Yamamuro, H. Kayanne, and I. Koike. 2002. Fine scale mapping of land-derived nitrogen in coral reefs by δ15N in macroalgae. Limnology and Oceanography 47: 1405–1416.CrossRefGoogle Scholar
  44. United States Environmental Protection Agency (USEPA) 2010. Watershed Assessment, Tracking, and Environmental Results. http://iaspub.epa.gov/waters10/attains_nation_cy.control#total_assessed_waters. Accessed 1 May 2010.
  45. Yamahara, K.M., B.A. Layton, A.E. Santoro, and A.B. Boehm. 2007. Beach sands along the California coast are diffuse sources of fecal bacteria to coastal waters. Environmental Science & Technology 41: 4515–4521.CrossRefGoogle Scholar
  46. Yamahara, K.M., S.P. Walters, and A.B. Boehm. 2009. Growth of enterococci in unaltered, unseeded beach sands subjected to tidal wetting. Applied and Environmental Microbiology 75: 1517–1524.CrossRefGoogle Scholar

Copyright information

© Coastal and Estuarine Research Federation 2010

Authors and Affiliations

  • Alexandria B. Boehm
    • 1
  • Kevan M. Yamahara
    • 1
  • Sarah P. Walters
    • 1
  • Blythe A. Layton
    • 4
  • Daniel P. Keymer
    • 1
  • Rachelle S. Thompson
    • 1
  • Karen L. Knee
    • 2
  • Matt Rosener
    • 3
  1. 1.Environmental and Water Studies, Department of Civil and Environmental EngineeringStanford UniversityStanfordUSA
  2. 2.Geological and Environmental SciencesStanford UniversityStanfordUSA
  3. 3.The Waipā FoundationKauaiUSA
  4. 4.Southern California Coastal Research ProjectCosta MesaUSA

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