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American Journal of Potato Research

, Volume 97, Issue 1, pp 78–87 | Cite as

The Opposite Effect of Low Temperature on the Pho1a Starch Phosphorylase Gene Expression in Solanum tuberosum L. Tubers and Petota Species Leaves

  • Maria A. SluginaEmail author
  • Alexey A. Meleshin
  • Elena Z. Kochieva
  • Anna V. Shchennikova
Article
  • 54 Downloads

Abstract

Starch turnover is important for plant response to abiotic stresses and plastidic starch phosphorylase Pho1a is a key enzyme regulating starch metabolism. In this study, we identified Pho1a coding sequences in seven wild tuber-bearing potato species. In Solanum tuberosum cv. Nadezhda grown at normal conditions, Pho1a transcription was low; it was detected in stems, leaves, flowers, and roots. After harvest, Pho1a expression in tubers of 16 S. tuberosum cultivars was either absent or low but progressively increased during storage at +4 °C, which corresponded to an increase in the content of reducing sugars. In the leaves of both cultivated and wild potato species, exposure to short-term cold stress downregulated Pho1a expression, especially at night, and stimulated starch degradation, but there was no uniform diurnal pattern in Pho1a expression dynamics. These findings indicate that Pho1a may have different functions in storage and photosynthetic organs of potato.

Keywords

Starch metabolism Wild potato species Plastidial starch phosphorylase Gene expression Cold stress Diurnal rhythm 

Resumen

La renovación del almidón es importante para la respuesta de la planta a los agobios abióticos, y la fosforilasa plastídica del almidón Pho1a es una enzima clave para la regulación del metabolismo del almidón. En este estudio, identificamos secuencias de codificación de Pho1a en siete especies silvestres tuberíferas de papa. En Solanum tuberosum var. Nadezhda cultivada bajo condiciones normales, la transcripción de Pho1a fue baja; se detectó en tallos, hojas, flores y raíces. Después de la cosecha, la expresión de Pho1a en tubérculos de 16 variedades de S. tuberosum estuvo ausente o fue baja, pero aumentó progresivamente durante el almacenamiento a + 4 °C, lo que correspondió a un aumento en el contenido de azúcares reductores. En las hojas, tanto de las especies cultivadas como en las silvestres, expuestas a cortos períodos de agobio por frío, la expresión de Pho1a estuvo regulada a la baja, especialmente en la noche, y estimuló la degradación del almidón, pero no hubo un patrón diurno uniforme en la dinámica de expresión de la Pho1a. Estos nuevos conocimientos indican que Pho1a puede tener diferentes funciones en almacén y en los órganos fotosintéticos de la papa.

Notes

Acknowledgments

This work was supported by the Russian Foundation for Basic Research (grants No. 17-29-08017 and 18-29-07007), the Ministry of Science and Higher Education of the Russian Federation and FSTP of Agricultural Development in the RF for 2017 - 2025 (subprogram “Development of potato breeding and seed production in the Russian Federation”), and was performed using the experimental climate control facility (Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences). We thank Dr. Marina Chuenkova for assistance in professional editing of the English.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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ESM 1 (XLS 520 kb)

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Copyright information

© The Potato Association of America 2019

Authors and Affiliations

  • Maria A. Slugina
    • 1
    Email author
  • Alexey A. Meleshin
    • 2
  • Elena Z. Kochieva
    • 1
  • Anna V. Shchennikova
    • 1
  1. 1.Institute of Bioengineering, Research Center of BiotechnologyRussian Academy of SciencesMoscowRussia
  2. 2.Lorch Potato Research Institutepos. KraskovoRussia

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